Iranian Journal of Veterinary Surgery

Iranian Journal of Veterinary Surgery

Influence of Ferula assa-foetida Loaded Chitosan Nanoparticle Biofilm on Wound Healing in Full-Thickness Wounds Infected with Methicillin Resistant Staphylococcus aureus

Document Type : Original Article

Authors
Mohammad-Ali Sadeghi 1
Shayan Kalantari 1
Fatemeh-Zahra Gharib 1
Firouz Faed-Maleki 2
Alireza Yousefi 1
1 Department of Veterinary Clinical Sciences, Faculty of Veterinary Medicine, Babol Branch, Islamic Azad University, Babol, Iran
2 Department of pharmacology, Faculty of Veterinary Medicine, Babol Branch, Islamic Azad University, Babol, Iran
10.30500/ivsa.2019.201547.1200
Abstract
Objective- Cutaneous wound healing is an essential physiological process consisting of the collaboration of many cell strains and their products. Initiation of new management for treatment of wound infections caused by multidrug resistant Staphylococcus aureus is required. The aim of the present study was to assess wound healing activity of Ferula assa-foetida loaded chitosan nanoparticle biofilm in methicillin resistant S. aureus (MRSA) infected wounds in rats.
Design- Experimental study
Animals- Forty eight male healthy Wistar rats.
Procedures- The animals were randomized into four groups of 12 animals each. In group I, the wounds were infected with MRSA and only treated with 0.1 mL the sterile saline 0.9% solution. In group II, the infected wounds were dressed with chitosan nanoparticles biofilm. In group III, animals with infected wounds were treated with 0.1 mL topical application of Ferula assa-foetida. In group IV, animals with infected wounds were dressed with Ferula assa-foetida loaded chitosan nanoparticles biofilm.
Results- Microbiological examination, planimetric, biomechanical, histological and quantitative morphometric studies and determination of hydroxyproline levels showed that there was significant difference between animals in group IV compared to other groups (p = 0.001).
Conclusion and Clinical Relevance- Ferula assa-foetida loaded chitosan nanoparticles biofilm could be useful for treatment of MRSA infected wounds in diabetes.
Keywords
MRSA
Ferula assafoetida
Chitosan nanoparticles biofilm
Infected wound

Subjects

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  • Receive Date 14 September 2019
  • Revise Date 02 November 2019
  • Accept Date 17 December 2019
  • First Publish Date 01 April 2020