Iranian Journal of Veterinary Surgery

Iranian Journal of Veterinary Surgery

Evaluation of the Anesthetic and Tranquilizing Effects of Clove Powder (Syzygium aromaticum) and Lavender Oil (Lavandula officinalis) in Convict Cichlid Fish (Cichlasoma nigrofasciata)

Document Type : Original Article

Authors
Abbas Raisi 1
Farshid Davoodi 2
Soroush Afshar-Ghahremani 2
Shayan Taheri 2
Siavash Sharifi 3
Milad Adel 4
1 Department of Clinical Studies, Faculty of Veterinary Medicine, Lorestan University, Khorram Abad, Iran
2 Student of Veterinary Medicine, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran
3 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
4 Department of Aquatic Animal Health & Disease, Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran
10.30500/ivsa.2019.193913.1191
Abstract
Objective- The present study describes the anesthetic effects of essential oil of Lavender (Lavandula officinalis) in Convict cichlid fish (Cichlasoma nigrofasciata) in comparison with clove powder (Syzygium aromaticum).
Study Design- experimental study
Animals- 25 Juvenile Convict cichlid fish with unknown sex and an average weight of 4.2 ± 0.5 g were used (n = 5 for each experimental group per experiment).
Methods- The fish were divided into five groups, initially with five fish per group. Each group was subjected to one of four final concentrations [0.3 and 0.5 g/l] of clove powder and [1.8 and 3 cc/l] essential oil of Lavender and control group was for determination of the basal values for blood parameters. The times for sedation, pre-anesthesia, and anesthesia were recorded based on the behavioral events seen after exposing the fish to each aquarium. After induction of anesthesia, the fish were transferred to anesthetic-free aquariums, and recovery time was recorded. Blood samples were taken by dissecting peduncle just 30 minutes after fish were placed in water contain anesthetics.
Results- essential oil of Lavender had efficacy to anesthetize the fish, and its sedation was similar to the clove powder at a commensurate concentration in Convict cichlid fish. However, the anesthesia form was different.
Conclusions and Clinical Relevance- Lavender oil as a sedative and anesthetic agent can be used in fish
Keywords
Lavender
Clove
Convict cichlid fish
Anesthesia
Hematological changes

Subjects

  1. Weber RA, Peleteiro JB, Martín LG, Aldegunde M. The efficacy of 2-phenoxyethanol, metomidate, clove oil and MS-222 as anaesthetic agents in the Senegalese sole (Soleasene galensis Kaup 1858). Aquaculture, 2009; 288(1-2): 147-150.
  2. Summerfelt RC. Anesthesia, surgery, and related techniques. Methods for fish biology, 1990.
  3. Pirhonen J, Schreck CB. Effects of anaesthesia with MS-222, clove oil and CO2 on feed intake and plasma cortisol in steelhead trout (Oncorhynchus mykiss). Aquaculture, 2003; 220(1-4): 507-514.
  4. Stickney RR. Encyclopedia of aquaculture. Awiley –interseience publication 2000; pp.34-37.
  5. Bagheri T, Imanpour MR. The efficacy, physiological responses and hematology of Persian Sturgeon, Acipenser persicus, to clove oil as an anesthetic agent. Turkish Journal of Fisheries and Aquatic Sciences, 2011; 11(3): 477-483.
  6. Balamurugan J, Kumar TT, Prakash S, Meenakumari B, Balasundaram C, Harikrishnan R. Clove extract: A potential source for stress free transport of fish. Aquaculture, 2016; 454: 171-175.
  7. Grush J, Noakes DL, Moccia RD. The efficacy of clove oil as an anesthetic for the zebrafish, Danio rerio (Hamilton). Zebrafish, 2004; 1(1): 46-53.
  8. Wimalasena SH, Pathirana HN, De Silva BC, Hossain S, Heo GJ. Antimicrobial activity of lavender (Lavendula rangustifolia) oil against fish pathogenic bacteria isolated from cultured olive flounder (Paralichthys olivaceus) in Korea. Indian Journal of Fisheries, 2018; 65(3): 52-56.
  9. McFarland WN. The use of anaesthetics for the handling and the transport of fishes. California Fish and Game. 1960; 46(4): 407-431.
  10. Larsen HN, Snieszko SF. Modification of the microhematocrit technique with trout blood. Transactions of the American Fisheries Society, 1961; 90(2): 139-142.
  11. Oser, BL. Blood analysis. Hawk's physiological chemistry,1965; p.1096.
  12. Weinbersg R, Siegelw CB, Nigrellain F, Gordon DAS. Weinberg SR, CD S, RF N, AS G. The hematological parameters and blood cell morphology of the brown bullhead catfish, Ictalurus nebulosus (Le Sueur). Zoologica, 1972; 57(9): 1-78.
  13. Wedemeyer GA, Yasutake WT. Clinical methods for the assessment of the effects of environmental stress on fish health. US Fish and Wildlife Service; 1977.
  14. Ross LG, Ross B. Anaesthetic and sedative techniques for aquatic animals. John Wiley & Sons; 2009.
  15. Woody CA, Nelson J, Ramstad K. Clove oil as an anaesthetic for adult sockeye salmon: field trials. Journal of Fish Biology, 2002; 60(2): 340-347.
  16. Soto CG. Clove oil as a fish anaesthetic for measuring length and weight of rabbitfish (Siganus lineatus). Aquaculture, 1995; 136(1-2): 149-152.
  17. Fischer IU, Von Unruh GE, Dengler HJ. The metabolism of eugenol in man. Xenobiotica, 1990; 20(2): 209-222.
  18. Liu EH, Gibson DM. Visualization of peroxidase isozymes with eugenol, a noncarcinogenic substrate. Analytical Biochemistry, 1977; 79(1-2): 597-601.
  19. Allen JL, Hunn JB. Fate and distribution studies of some drugs used in aquaculture. Veterinary and Human Toxicology. 1986; 28: 21-24.
  20. Burka JF, Hammell KL, Horsberg TE, Johnson GR, Rainnie DJ, Speare DJ. Drugs in salmonid aquaculture–a review. Journal of veterinary Pharmacology and Therapeutics. 1997; 20(5): 333-349.
  21. Wagner GN, Singer TD, McKinley RS. The ability of clove oil and MS‐222 to minimize handling stress in rainbow trout (Oncorhynchus mykiss Walbaum). Aquaculture Research. 2003; 34(13): 1139-1146.
  22. Holloway AC, Keene JL, Noakes DG, Moccia RD. Effects of clove oil and MS‐222 on blood hormone profiles in rainbow trout Oncorhynchus mykiss, Walbaum. Aquaculture Research, 2004; 35(11): 1025-1030.
  23. Hajek GJ, Klyszejko B, Dziaman R. The anaesthetic effects of clove oil on common carp, Cyprinus carpio L. Acta Ichthyologica et Piscatorial, 2006; 2(36): 93-97.
  24. Gomulka P, Własow T, Velíšek J, Svobodová Z, Chmielinska E. Effects of eugenol and MS-222 anaesthesia on Siberian sturgeon Acipenserbaerii brandt. Acta Veterinaria Brno, 2008; 77(3): 447-453.
  25. Alnamer R, Alaoui K, Bouidida EH, Benjouad A, Cherrah Y. Sedative and hypnotic activities of the methanolic and aqueous extracts of Lavandula officinalis from Morocco. Advances in Pharmacological Sciences, 2012.
  26. Bradley BF, Starkey NJ, Brown SL, Lea RW. Anxiolytic effects of Lavandula angustifoliaodour on the Mongolian gerbil elevated plus maze. Journal of Ethnopharmacology, 2007; 111(3): 517-525.
  27. Lis‐Balchin M, Hart S. Studies on the mode of action of the essential oil of Lavender Lavandula angustifolia P. Miller. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives, 1999; 13(6): 540-542.
  28. Sudagara M, Mohammadizarejabada A, Mazandarania R, Pooralimotlagha S. The efficacy of clove powder as an anesthetic and its effects on hematological parameters on roach (Rutilus rutilus). Journal of Aquaculture Feed Science and Nutrition, 2009; 1(1): 1-5.
  29. Iwama GK, McGeer JC, Pawluk MP. The effects of five fish anaesthetics on acid–base balance, hematocrit, blood gases, cortisol, and adrenaline in rainbow trout. Canadian Journal of Zoology, 1989; 67(8): 2065-2073.
  30. Bolasina SN. Cortisol and hematological response in Brazilian codling, Urophycis brasiliensis (Pisces, Phycidae) subjected to anesthetic treatment. Aquaculture International, 2006; 14(6): 569-575.
  31. Limsuwan C, Limsuwan T, Grizzle JM, Plumb JA. Stress response and blood characteristics of channel catfish (Ictalurus punctatus) after anesthesia with etomidate. Canadian Journal of Fisheries and Aquatic Sciences, 1983; 40(12): 2105-2112.

  • Receive Date 11 July 2019
  • Revise Date 20 August 2019
  • Accept Date 26 October 2019
  • First Publish Date 01 April 2020