Document Type : Original Article
Authors
- Elnaz Azad 1
- Farajollah Adibhashemi 1
- Mohammad Mehdi Dehghan 1
- Seyed Mehdi Ghamsari 2
- Reza Sedaghat 3
- Seyed Mehdi Nasiri 4
- Pegah Abbasnia
1 Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
2 Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
3 Department of Pathology, Shahed University of Medical Sciences, Tehran, Iran
4 Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Abstract
Objective- To evaluate growth characteristics and differentiation capacity of equine mesenchymal stem cell (eMSCs) derived from bone marrow (BM).
Study design- In vitro experimental study.
Animals- Four young adult horses (2-5 years old)
Procedure- Cell morphology and growth characteristics of eMSCs harvested from BM were evaluated in standard culture conditions. eMSCs in passage 3 were subjected to osteogenic and adipogenic differentiation induction to investigate their differentiation potential.PCR analysis of differentiated cells was done to determine differentiation.
Results-The cells expanded to sufficient quantity for therapeutic purposes within days and they survived to later passages while sustained their fibroblast-like morphology. Positive osteogenesis was detected via Alizarin Red staining and adipogenesis was confirmed by Oil Red O staining. The cells were more potential to differentiate into osteoblasts rather than adipocytes. PCR analysis approve relative expression of osteogenic and adipogenic genes.
Conclusions and Clinical relevance - The results of this study further support eBM-MSCs as a cell population with the capacity to proliferate and differentiate down the osteogenic and adipogenic lineages. Equine BM is a potentials source of MSCs for cell-based regenerative therapies in horse.
Keywords
Main Subjects
)