Iranian Journal of Veterinary Surgery

Iranian Journal of Veterinary Surgery

Effects of Fisetin on Cutaneous Full Thickness Wound Healing in a Rat Model

Document Type : Original Article

Authors
Behdad Beheshti Alagha 1
Farshid Davoodi 2
Rahim Mohammadi 3
Seyede Soraya Mahmoudi 4
1 Department of Veterinary Medicine, Karaj Branch, Islamic Azad University, Karaj, Iran.
2 Department of Clinical Sciences, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran.
3 Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
4 Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
10.30500/ivsa.2025.519077.1444
Abstract
Cutaneous wound healing is a complex biological process involving inflammation, proliferation, and tissue remodeling. Fisetin, a dietary flavonoid found in fruits and vegetables, is known for its potent antioxidant and anti-inflammatory properties. However, its therapeutic potential in wound healing has not been previously explored. This study aimed to evaluate the effects of topical fisetin on skin wound healing in a rat model. Forty male rats were randomly divided into four groups: control (no treatment), olive oil (vehicle), treatment 1 (T1; 10 mg/kg fisetin in olive oil), and treatment 2 (T2; 20 mg/kg fisetin in olive oil). A full-thickness dorsal wound (20 mm) was created in each rat. Treatments were administered topically for 14 days. Wound contraction was measured on days 3, 6, 9, 12, and 14. Oxidative stress markers malondialdehyde (MDA), Total oxidant status (TOS), superoxide dismutase (SOD), and total antioxidant capacity (TAC) were assessed on day 14, and histological evaluation using H&E staining was performed on days 7 and 14. Fisetin significantly accelerated wound contraction, particularly in the T2 group. MDA and TOS levels were significantly reduced, while SOD and TAC were elevated in fisetin-treated groups compared to controls. Histopathological analysis revealed reduced inflammatory cell infiltration, enhanced collagen deposition, and re-epithelialization, especially in the T2 group. Topical application of fisetin markedly improves cutaneous wound healing by modulating oxidative stress and enhancing tissue regeneration. These findings suggest fisetin as a promising natural agent for promoting skin repair and warrant further investigation in clinical settings.
Keywords
Wound healing
Flavonoids
Oxidative stress
Skin
Fisetin
Rat

Subjects

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  • Receive Date 25 April 2025
  • Revise Date 16 May 2025
  • Accept Date 23 June 2025
  • First Publish Date 23 June 2025