Document Type : Original Article
Authors
1 Department of Internal Medicine and Clinical Pathology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
2 Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Abstract
Ischemia/reperfusion can cause tissue damage and affect organ function. Ischemia-reperfusion injury (IRI) occurs in a variety of clinical manifestations such as stroke, trauma, and surgery. The present study investigated the role of pyrroloquinoline quinone (PQQ) on the renal damage after IRI. Rats were assigned to four groups randomly (n = 8): Control group, Sham group, Renal IRI (45 minutes ischemia and subsequently 24 hours reperfusion) and renal IRI + PQQ (10 mg/kg /IP). Serum level of urea, creatinine, TNF-α and IL-6 were measured by a biochemical analyzer using commercial kits. In kidney tissue samples total antioxidant capacity (TAC), malondialdehyde (MDA), and myeloperoxidase (MPO) were measured using commercial kits. The results showed that IRI injury increased serum urea, creatinine levels, TNF-α and IL-6 along with MDA, MPO levels in the renal tissue, and decreased renal TAC levels. A decrease in serum urea, creatinine levels as well as MDA, MPO levels of the IR + PQQ group were observed in renal tissues. In addition, TAC levels in the kidney tissues of PQQ-treated animals were improved in comparison with the IRI group. In conclusion, pyrroloquinoline quinone treatment ameliorated renal IR injury by anti-oxidative stress and anti-inflammatory properties. Consequently, it could be promising as a potential therapeutic agent for renal ischemia-reperfusion injury.
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- Malek M, Nematbakhsh M. Renal ischemia/reperfusion injury; from pathophysiology to treatment. Journal of Renal Injury Prevention. 2015; 4: 20.
- Zhang J, Tang L, Li GS, Wang J. The anti-inflammatory effects of curcumin on renal ischemia-reperfusion injury in rats. Renal Failure. 2018; 40: 680-686.
- Hashmp SF, Sattar MZ, Rathore HA, Ahmadi A, Johns EJ. A critical review on pharmacological significance of hydrogen sulfide (h (2) s) on NF-kappab concentration and icam-1 expression in renal ischemia reperfusion injury. Acta Poloniae Pharmaceutica. 2017; 74: 747-752.
- Salvadori M, Rosso G, Bertoni E. Update on ischemia-reperfusion injury in kidney transplantation: Pathogenesis and treatment. World Journal of Transplantation. 2015; 5: 52.
- Carden DL, Granger D. Pathophysiology of ischaemia–reperfusion injury. Journal of Patholology. 2000; 190: 255-266.
- Akagawa M, Nakano M, Ikemoto K. Recent progress in studies on the health benefits of pyrroloquinoline quinone. Bioscience, Biotechnology, and Biochemistry. 2016; 80(1): 13-22.
- Jonscher KR, Chowanadisai W, Rucker R. Pyrroloquinoline-quinone is more than an antioxidant: a vitamin-like accessory factor important in health and disease prevention. Biomolecules. 2021; 11: 1441.
- Parhizkar P, Mohammadi R, Shahrooz R, Mohammadi V. Effects of pyrroloquinoline quinone (PQQ) on ischemia-reperfusion injury in rat ovaries: histological and biochemical assessments. Bulletin of Emergency and Trauma. 2019; 7: 35.
- Zhu BQ, Zhou HZ, Teerlink JR, Karliner JS. Pyrroloquinoline quinone (PQQ) decreases myocardial infarct size and improves cardiac function in rat models of ischemia and ischemia/reperfusion. Cardiovascular Drugs and Therapy. 2004; 18: 421-431.
- Lu H, Shen J, Song X, Ge J, Cai R, Dai A, Jiang Z. Protective effect of pyrroloquinoline quinone (PQQ) in rat model of intracerebral hemorrhage. Cellular and Molecular Neurobiology. 2015; 35:.921-930.
- Wu Y, Zhao M, Lin Z. Pyrroloquinoline quinone (PQQ) alleviated sepsis-induced acute liver injury, inflammation, oxidative stress and cell apoptosis by downregulating CUL3 expression. Bioengineered, 2021; 12(1):.2459-2468.
- Nezamoleslami S, Sheibani M, Dehpour AR, Mobasheran P, Shafaroodi H. Glatiramer acetate attenuates renal ischemia reperfusion injury in rat model. Experimental and Molecular Pathology. 2020; 112: 104329.
- Nezamoleslami S, Sheibani M, Jahanshahi F, Mumtaz F, Abbasi A, Dehpour AR. Protective effect of dapsone against renal ischemia-reperfusion injury in rat. Immunopharmacology and Immunotoxicology. 2020; 42: 272-279.
- Tian Y, Shu J, Huang R, Chu X, Mei X. Protective effect of renal ischemic postconditioning in renal ischemic-reperfusion injury. Translational Andrology and Urology. 2020; 9: 1356.
- Chen H, Xing B, Liu X, Zhan B, Zhou J, Zhu H, Chen Z. Ischemic postconditioning inhibits apoptosis after renal ischemia/reperfusion injury in rat. Transplant International. 2008; 21: 364-371.
- Pulli B, Ali M, Forghani R, Schob S, Hsieh KLC, Wojtkiewicz G, Linnoila JJ, Chen JW. Measuring myeloperoxidase activity in biological samples. PLoS One. 2013; 8: e67976.
- Gao Y, Huang Y, Zhao Y, Hu Y, Li Z, Guo Q, Zhao K, Lu N. LL202 protects against dextran sulfate sodium-induced experimental colitis in mice by inhibiting MAPK/AP-1 signaling. Oncotarget. 2016; 7: 63981.
- Jang HR, Ko GJ, Wasowska BA, Rabb H. The interaction between ischemia–reperfusion and immune responses in the kidney. Journal of Molecular Medicine. 2009; 87: 859-864.
- Liu J, Kumar S, Dolzhenko E, Alvarado GF, Guo J, Lu C, Chen Y, Li M, Dessing MC, Parvez RK, Cippà PE, Krautzberger AM, Saribekyan G, Smith AD, McMahon AP. Molecular characterization of the transition from acute to chronic kidney injury following ischemia/reperfusion. JCI Insight. 2017; 2.
- Bonventre JV, Yang L. Cellular pathophysiology of ischemic acute kidney injury. Journal Clincal Investigation. 2011; 121: 4210-4221.
- Jiang G, Wang M, Wang L, Chen H, Chen Z, Guo J, Weng X, Liu X. The protective effect of nesfatin-1 against renal ischemia–reperfusion injury in rats. Renal Failure. 2015; 37: 882-889.
- Sirotković-Skerlev M, Plestina S, Bilić I, Kovac Z. Pathophysiology of ischaemia-reperfusion injury. Lijecnicki Vjesnik. 2006; 128: 87-95.
- Hong X, Zhao X, Wang G, Zhang Z, Pei H, Liu Z. Luteolin treatment protects against renal ischemia-reperfusion injury in rats. Mediators of Inflammation. 2017; 2017: 9783893.
- Shi N, Wu MP. Apolipoprotein AI attenuates renal ischemia/reperfusion injury in rats. Journal Biomedical Science. 2008; 15(5): 577-583.
- Dejban P, Rahimi N, Takzare N, Jahansouz M, Haddadi N-S, Dehpour AR. Beneficial effects of dapsone on ischemia/reperfusion injury following torsion/detorsion in ipsilateral and contralateral testes in rat. Theriogenology. 2019; 140: 136-142.
- Sheibani M, Nezamoleslami S, Faghir-Ghanesefat H, Dehpour AR,. Cardioprotective effects of dapsone against doxorubicin-induced cardiotoxicity in rats. Cancer Chemotherapy Pharmacology. 2020; 85: 563-571.
- Lawrence T. The nuclear factor NF-κB pathway in inflammation. Cold Spring Harbor Perspectives in Biology. 2009; 1: a001651.
- Daemen MA, de Vries B, Buurman W. Apoptosis and inflammation in renal reperfusion injury. Transplantation. 2002; 73: 1693-1700.
- Yang K, Li WF, Yu JF, Yi C, Huang W. Diosmetin protects against ischemia/reperfusion-induced acute kidney injury in mice. Journal Surgical Research. 2017; 214: 69-78.
- Donnahoo KK, Meldrum DR, Shenkar R, Chung CS, Abraham E, Harken AH. Early renal ischemia, with or without reperfusion, activates NFκB and increases TNF-α bioactivity in the kidney. Journal Urology. 2000; 163: 1328-1332.
- Liu YX, Jin LM, Zhou L, Xie HY, Jiang GP, Wang Y, Feng XW, Chen H, Yan S, Zheng SS. Mycophenolate mofetil attenuates liver ischemia/reperfusion injury in rats. Transplant International. 2009; 22: 747-756.
- Gaweł S, Wardas M, Niedworok E, Wardas P. Malondialdehyde (MDA) as a lipid peroxidation marker. Wiadomosci Lekarskie. 2004; 57: 453-455.
- Inal M, Altinişik M, Bilgin MD. The effect of quercetin on renal ischemia and reperfusion injury in the rat. Cell Biochemistry and Function: Cellular Biochemistry and its Modulation by Active Agents or Disease. 2002; 20: 291-296.
- Wu K, Li H, Tian J, Lei W. Protective effect of baicalein on renal ischemia/reperfusion injury in the rat. Renal Failure. 2015; 37: 285-291.
- Malle E, Buch T, Grone H-J. Myeloperoxidase in kidney disease. Kidney International. 2003; 64: 1956-1967.
- Heinzelmann M, Mercer-Jones MA, Passmore JC. Neutrophils and renal failure. American Journal of Kidney Diseases. 1999; 34: 384-399.