Iranian Journal of Veterinary Surgery

Iran J Vet Surg, Print ISSN: 2008-3033, Online ISSN: 2676-6299

Current Issue

By Issue

By Author

By Subject

Author Index

Keyword Index

About Journal

Aims and Scope

Publication Ethics

Peer Review Process

Editorial Board

Indexing and Abstracting

Related Links

News

The Effects of Honey, Curcumin, Ginger, Nigella sativa and Their Mixture on the Radial Bone Defect Repair in Rat Model: Histological and Biomechanical Study

Document Type : Original Article

Authors

Department of Surgery, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.

Abstract

Honey, curcumin, ginger, and Nigella sativa are known for their antioxidant, immune stimulation properties, and wound healing effects. This study was conducted to determine the effect of these compounds and their mixture on the bone healing of the radial bone defect model in rats. One hundred and ten radial bone defects in 55 healthy male rats were randomly divided into 11 groups (10 rats per group). Groups 1-5 received honey, curcumin, ginger, and Nigella sativa orally by gavage, and groups 6-10 received the same materials locally by rubbing them on the sutured skin. The bone repair effects of these materials were evaluated by histopathology and biomechanical examinations. Using honey, curcumin, ginger, and Nigella sativa exhibited a beneficial effect to promote bone healing in this study. The biomechanical and histopathologic evaluations showed that the honey (oral), the mixture of honey and curcumin (oral), and the mixture of honey and Nigella sativa (topical) groups exhibited significantly better results on the 52nd postoperative day compared to the other groups.

Keywords

Main Subjects

References
  1. Ferguson C, Alpern E, Miclau T, Helms JA. Does adult fracture repair recapitulate embryonic skeletal formation? Mechanisms of Development. 1999; 87: 57-66.
  2. Carter DR, Van der Meulen MC, Beaupre GS. Mechanical factors in bone growth and development. Bone. 1996; 18S: 5-10.
  3. Rockwood Jr CA, Green DP, Bucholz RW, Heckman JD. Fractures in adults, 4th, Philadelphia: Lippincott, USA, 1996.
  4. Molan PC. The role of honey in the management of wounds. Journal of wound care. 1999; 8(8): 415–418.
  5. Al-Waili NS, Salom K, Butler G, Al Ghamdi AA. Honey and microbial infections: A review supporting the use of honey for microbial control. Journal of Medicinal Food. 2011; 14(10): 1079–1096.
  6. Effendy NM, Norazlina M, Norliza M, Mohamad IN, Shuid AN. The effects of Tualang honey on bone metabolism of postmenopausal women. Evidence Based Complement Alternat Medicine. 2012; 93-98.
  7. Shukla Y, Singh M. Cancer preventive properties of ginger: A brief review. Food Chemical Toxicol 2007; 45: 683–690.
  8. Jiang H, Xie Z, Koo HJ, McLaughlin SP, Timmermann BN, Gang DR. Metabolic profiling and phylogenetic analysis of medicinal Zingiber species: Tools for authentication of ginger (Zingiber officinale Rosc.) Phytochemistry. 2006; 67:232–244.
  9. Ali BH, Blunden G, Tanira MO, Nemmar A. Some phytochemical, pharmacological and toxicological properties of ginger (Zingiber officinale Roscoe): a review of recent research. Food Chemical Toxicology. 2008; 46: 409–420.
  10. Panchatcharam M, Miriyala S, Gayathri VS, Suguna L. Curcumin improves wound healing by modulating collagen and decreasing reactive oxygen species. Molecular Cell Biochemistry. 2006; 290(1–2): 87–96.
  11. Dae-Chul K, Tae K, Younghoon J, Joo-Kyung S. A synergistic bone sparing effect of Curcumin and Alendronate in ovariectomized rat. Acta Neurochirurgica. 2012; 154: 2215–2223.
  12. French DL, Muir JM, Webber CE. The ovariectomized, mature rat model of postmenopausal osteoporosis: an assessment of the bone sparing effects of Curcumin. Phytomedicine. 2008; 15 (12): 1069–1078.
  13. Bharti AC, Takada Y, Aggarwal BB. Curcumin (diferuloylmethane) inhibits receptor activator of NF-kB ligand-induced NF-kappa B activation in osteoclast precursors and suppresses osteoclastogenesis. Journal of Immunology. 2004; 172(10): 5940–5947.
  14. Ezirganli S, Kazancioglu HO, Ozdemir H, Inan DS, Tek M. The effects of Nigella sativa Seed extract on bone healing in an experimental model. Journal of Craniofacial Surgery. 2016; 27(7): 1905-1909.
  15. Cheng C, Alt V, Dimitrakopoulou-Strauss A, Pan L, Thormann U, Schnettler R, Weber K, Strauss LG. Evaluation of new bone formation in normal and osteoporotic rats with a 3-mm femur defect: functional assessment with dynamic PET-CT (dPET-CT) using 2-deoxy-2-[(18)F]fluoro-D-glucose ((18)F-FDG) and (18)F-fluoride. Molecular Imaging Biol 2013; 15(3): 336-344.
  16. Mukhopadhyay MJ, Saha A, Mukherjee A. Studies on the clastogenic effect of turmeric and curcumin on cyclophosphamide and mitomycin in vivo, Food Chemistry Toxicol 1998; 36: 73–76.
  17. Tamer A. Gheita, S, Kenawy A. Effectiveness of Nigella sativa Oil in the management of rheumatoid arthritis patients: A Placebo controlled study. Phytotherapy Research. 2011; 10: 1002-1009.
  18. Weidnera MS, Sigwart K. Investigation of the teratogenic potential of a Zingiber officinale extract in the rat. Reproductive Toxicology. 2001; 15: 75–80.
  19. Lusania M. Greggi A, Cristina M, Araujo P, D’Arc J, Darin C, deLourdes M, Bianchi p. Effects of the antioxidants curcumin and vitamin C on cisplatin-induced clastogenesis in Wistar rat bone marrow cells. Mutation Research. 2000; 465: 131–137.
  20. Matthew P, Brennan MD, Albert J, Sinusas MD, Tamas L, Horvath DVM, Collins JG, Martha J. Harding, DVM. Correlation between body weight changes and postoperative pain in rats treated with meloxicam or buprenorphine. Journal of Lab Animal. 2009; 38(3): 87–93.
  21. Slate AR, Bandyopadhyay S, Francis KP, Papich MG, Karolewski B, Hod EA, Prestia KA. Efficacy of Enrofloxacin in a Mouse Model of Sepsis. Journal of American Association for Lab Animal Science. 2014; 53(4): 381–386.
  22. Tasker L, Methods for the euthanasia of dogs and cats: comparison and recommendations. World Society for Protection of animals. London. 2008.
  23. Oryan A, Alidadi S, Bigham-Sadegh A, Meimandi-Parizi A. Chitosan/gelatin/platelet gel enriched by a combination of hydroxyapatite and beta-tricalcium phosphate in healing of a radial bone defect model in rat, International Journal of Biological Macromolecule. 2017; 101: 630–637.
  24. Oryan A, Alidadi S, Bigham-Sadegh A, Moshiri A. Comparative study on the role of gelatin, chitosan and their combination as tissue engineered scaffolds on healing and regeneration of critical sized bone defects: an in vivo study. Journal of Material Science Material Medicine. 2016; 27: 155-161.
  25. Oryan A, Bigham S, Teshnizi F. Effects of osteogenic medium on healing of the experimental critical bone defect in a rabbit model. Journal of bone. 2008; 63: 53-60.
  26. Hajizadeh F, Derakhshan B, Peimani A, Abbasi Z. Effect of Topical Honey on Mandibular Bone Defect Healing in Rats. Journal of Contemporary Dental Practice. 2018; 19(1): 47-51.
  27. Bigham A, Oryan A, Karimi I, Hossini F, Sharifi S, Pakzad A. Effects of honey and hydroxyapatite on bone healing in rats. Journal Trauma Monthly. 2018; 23(4): 561-569.
  28. Efem SE. Clinical observations on the wound healing properties of honey. British Journal of Surgery. 1988; 75(7): 679-681.
  29. Weston RJ. The contribution of catalase and other natural products to the antibacterial activity of honey: a review. Food Chemistry. 2000; 71(2): 235-239.
  30. Singh VV, Pal US, Singh R, Soni N. Honey a sweet approach to alveolar osteitis: a study National Journal Maxillofac Surgery. 2014; 5(1): 31-34.
  31. Molan PC. The antibacterial activity of honey. 1. The nature of the antiabcterial activity. Bee World. 1992; 73(1): 5-28.
  32. Sazegar G, Seyed Reza AH, Behravan E. The effects of supplemental zinc and honey on wound healing in rats. Iran Journal of Basic Medical Science. 2011; 145(1): 3-9.
  33. Namias N. Honey in the management of infections. Journal of Surgery Infect (Larchmt). 2003; 4(2): 219-226.
  34. Fournier P, Dupius Y. Modulation of intestinal absorption of calcium. Journal of Physiology. 1975; 70(4): 479-491.
  35. Ariefdjohan MW, Berdine RM, Lachcik PJ, Weaver CM. Acute and chronic effects of honey and its carbohydrate constituents on calcium absorption in rats. Journal of Agriculture Food Chemistry. 2008; 56(8): 2649-2654.
  36. Trivedi R, Kumar S, Kumar A, Siddiqui JA, Swarnkar G, Gupta V, Kendurker A, Dwivedi AK, Romero JR, Kaempferol has osteogenic effect in ovariectomized adult Sprague-Dawley rats. Molecular Cellular Endocrinology. 2008; 289(1-2): 85-93.
  37. Moran JM, Roncero-Martin R, Francisco J. Rodriguez V, Julian F, Calderon G, Purification R, Vicente, Maria L. Canal M, Juan D, Pedrera Z, Effects of Curcumin on the Proliferation and Mineralization of Human Osteoblast-Like Cells: Implications of Nitric Oxide. International Journal of Molecular Sciences. 2012; 13(12): 16104–16118.
  38. Leung A. Encyclopedia of common natural ingredients used in food, drugs, and cosmetics. John Wiley, New York, NY. 1980; 313–314.
  39. Sidhu GS, Singh AK, Thaloor D, Banaudha KK, Patnaik GK, Srimal RC, Maheshwari RK. Enhancement of wound healing by curcumin in animals. Wound Repair and Regeneration 1998; 6(2): 167–177.
  40. Sidhu GS, Mani H, Gaddipati JP, Singh AK, Seth P, Banaudha KK, Patnaik GK, Maheshwari RK. Curcumin enhances wound healing in streptozotocin induced diabetic rats and genetically diabetic mice. Wound Repair and Regeneration. 1999; 7(5): 362–374.
  41. Kulac M, Aktas C, Tulubas F, Uygur R, Kanter M, Erboga M, Mehmet C, Topcu B, Aslan O, The effects of topical treatment with curcumin on burn wound healing in rats. Journal of Molecular Histology. 2013; 44(1): 83–90.
  42. Bartels EM, Folmer VN, Bliddal H, Altman RD, Juhl C, Tarp S, Zhang W, Christensen R. Efficacy and safety of ginger in osteoarthritis patients: A meta-analysis of randomized placebo-controlled trials. Osteoarthritis Cartilage. 2015; 23: 13–21.
  43. Rahnama P, Montazeri A, Huseini HF, Kianbakht S, Naseri M. Effect of Zingiber officinale R. rhizomes (ginger) on pain relief in primary dysmenorrhea: A placebo randomized trial. BMC Complement Alternative 2012; 12: 92-96.
  44. Terry R, Posadzki P, Watson LK, Ernst E. The use of ginger (Zingiber officinale) for the treatment of pain: A systematic review of clinical trials. Pain Medicine. 2011; 12: 1808–1818.
  45. Darvishzadeh-Mahani F, Esmaeili-Mahani S, Komeili G, Sheibani V, Zare L. Ginger (Zingiber officinale Roscoe) prevents the development of morphine analgesic tolerance and physical dependence in rats. Journal of Ethnopharmacology. 2012; 141: 901–907,
  46. Reid SA, Speedy DB, Thompson JM, Noakes TD, Mulligan G, Page T, Campbell RG, Milne C. Study of hematological and biochemical parameters in runners completing a standard marathon. Clinical Journal of Sport Medicine. 2004; 14: 344–353.
  47. Sepahvand R, Esmaeili-Mahani S, Arzi A, Rasoulian B, Abbasnejad M. Ginger (Zingiber officinale Roscoe) elicits antinociceptive properties and potentiates morphine-induced analgesia in the rat radiant heat tail-flick test. Journal of Medical Food. 2010; 13: 1397–1401.
  48. Ali BH, Blunden G, Tanira MO, Nemmar A. Some phytochemical, pharmacological and toxicological properties of ginger (Zingiber officinale Roscoe): a review of recent research. Food Chemical Toxicol 2008; 46: 409–420.
  49. Lantz RC, Chen GJ, Sarihan M, Solyom AM, Jolad SD, Timmermann BN. The effect of extracts from ginger rhizome on inflammatory mediator production. 2007; 14: 123–128.
  50. Qadir M, Imran B, Farhan M. Effect of ginger extract on angiogenesis using CAM assay. Bangladesh Journal of Pharmacol 2017; 12: 348-353.
  51. Bhagavathula N, Roscoe L. Warner M, DaSilva, BS, Shannon D. McClintock BS; Adam B, Muhammad N. Aslam MD, Kent J. Johnson MD, James, P. A combination of curcumin and ginger extract improves abrasion wound healing in corticosteroid-impaired hairless rat skin. Wound Repair and Regeneration, 2009; 17: 360–366.
  52. Dehghani SN, Bahrololum ME, Ghaderi AA, Ghadirian SB, Faghihi Z, Javidi M, Emami MJ. Hydroxyapatite stem cell repair of radial fracture gap in rabbits, Online Journal of Veterinary Research. 2017; 21(9): 616-619.
Iranian Journal of Veterinary Surgery
Volume 17, Issue 2 - Serial Number 37
October 2022
Pages 87-94
Files
History
  • Receive Date: 25 February 2022
  • Revise Date: 15 May 2022
  • Accept Date: 28 May 2022
  • First Publish Date: 30 May 2022
Share
How to cite
Statistics