Document Type : Original Article
Authors
- Mohammad Shahraki 1
- Mohammad Mahdi Molaei 1
- Reza Kheirandish 2
- Pourya Mohammadi 3
- Ehsanollah Sakhaee 4
1 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
2 Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
3 Department of Chemistry, Shahid Bahonar University of Kerman, Kerman, Iran
4 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University o Kerman, Kerman, Iran
Abstract
The present study was conducted to evaluate the wound healing effects of liposome nanocarrier containing Scrophularia striata extract. Seventy-two male Wistar albino rats were divided into 6 groups of 12 animals each. Rats were anesthetized, and dorsum shaved. A 100 g cylindrical copper rod of 1cm diameter was heated up to 100° C. It was placed on the skin without any pressure. Histopathological evaluation and the macroscopic size processing and analysis of the burn were employed to study the potential of wound healing in study groups. The study groups were: positive control group (zinc oxide treatment), treatment group (ointment containing nanoliposomes loaded with extract Scrophularia striata), extract group (ointment containing hydroalcoholic extract Scrophularia striata), control group of nanoliposomes (ointment containing Nano liposomes), Eucerin control group (ointment containing Eucerin), negative control group (no treatment). On the 5th, 10th, and 15th days after the initial operation, samples were taken from 4 rats of each group. The results showed that loaded nanoliposomes had a nanometric size and spherical morphology. Also, the wound size of the treatment group was smaller than other groups and had the best effect on repairing wound healing. Also, in other groups, positive control had a better effect on wound repair than extract, Eucerin, nano, and negative groups. According to the highest re-epithelialization, granulation tissue formation, and the lowest necrotic tissue, we could suggest that the treatment group had the best healing effect among other groups.
Keywords
Main Subjects
- Reinke J, Sorg H. Wound repair and regeneration. European Surgical Research. 2012;49(1):35-43.
- Agyare C, Dwobeng AS, Agyepong N, Boakye YD, Mensah KB, Ayande PG, Adarkwa-Yiadom M. Antimicrobial, antioxidant, and wound healing properties of Kigelia africana (Lam.) Beneth. and Strophanthus hispidus DC. Advances in Pharmacological Sciences. 2013.
- Chai J, Song H, Sheng Z, Chen B, Yang H, Li L. Repair and reconstruction of massively damaged burn wounds. Burns. 2003;29(7):726-732.
- Pruitt Jr BA, McManus AT, Kim SH, Goodwin CW. Burn wound infections: current status. World Journal of Surgery. 1998;22(2):135-145.
- Riegel RJ. Laser therapy for the treatment of equine wounds. Laser Therapy in Veterinary Medicine: Photobiomodulation. 2017:375.
- Goorani S, Zangeneh MM, Koohi MK, Seydi N, Zangeneh A, Souri N, Hosseini MS. Assessment of antioxidant and cutaneous wound healing effects of Falcaria vulgaris aqueous extract in Wistar male rats. Comparative Clinical Pathology. 2019;28(2):435-445.
- Ghashghaii A, Hashemnia M, Nikousefat Z, Zangeneh MM, Zangeneh A. Wound healing potential of methanolic extract of Scrophularia striata in rats. Pharmaceutical Sciences. 2017;23(4):256-263.
- Tanideh N, Haddadi MH, Rokni-Hosseini MH, Hossienzadeh M, Mehrabani D, Sayehmiri K, Koohi-Hosseinabadi O. The healing effect of scrophularia striata on experimental burn wounds infected to pseudomonas aeruginosa in rat. World Journal of Plastic Surgery. 2015;4(1):16.
- Wang W, Lu K-j, Yu C-h, Huang Q-l, Du Y-Z. Nano-drug delivery systems in wound treatment and skin regeneration. Journal of Nanobiotechnology. 2019;17(1):1-15.
- Souto EB, Ribeiro AF, Ferreira MI, Teixeira MC, Shimojo AA, Soriano JL, Naveros BC, Durazzo A, Lucarini M, Souto SB, Santini A. New nanotechnologies for the treatment and repair of skin burns infections. International Journal of Molecular Sciences. 2020;21(2):393.
- Li Z, Liu M, Wang H, Du S. Increased cutaneous wound healing effect of biodegradable liposomes containing madecassoside: preparation optimization, in vitro dermal permeation, and in vivo bioevaluation. International Journal of Nanomedicine. 2016;11:2995.
- Nasab ME, Takzaree N, Saffari PM, Partoazar A. In vitro antioxidant activity and in vivo wound-healing effect of lecithin liposomes: a comparative study. Journal of Comparative Effectiveness Research. 2019;8(8):633-643.
- Haddadi R, Tamri P, Jooni FJ. In vitro wound healing activity of Scrophularia striata hydroalcoholic extract. South African Journal of Botany. 2019;121:505-509.
- Noudoost B, Noori N, Amo Abedini G, Gandomi H, Akhondzadeh Basti A, Jebeli Javan A, Ghadami F. Encapsulation of green tea extract in nanoliposomes and evaluation of its antibacterial, antioxidant and prebiotic properties. Journal of Medicinal Plants. 2015;3(55):66-78.
- Golmohammadzadeh S, Jaafari M, Khalili N, Greenoak G. Determination of SPF and moisturizing effects of liposomal and conventional formulations of octyl methoxycinnamte as a sunscreen. Iranian Journal of Basic Medical Sciences. 2007;10(2):99-110.
- Asprea M, Tatini F, Piazzini V, Rossi F, Bergonzi MC, Bilia AR. Stable, monodisperse, and highly cell-permeating nanocochleates from natural soy lecithin liposomes. Pharmaceutics. 2019;11(1):34.
- Patel U, Kulkarni M, Undale V, Bhosale A. Evaluation of diuretic activity of aqueous and methanol extracts of Lepidium sativum garden cress (Cruciferae) in rats. Tropical Journal of Pharmaceutical Research. 2009;8(3).
- Cai EZ, Ang CH, Raju A, Tan KB, Hing ECH, Loo Y, Wong YC, Lee H, Lim J, Moochhala SM, Hauser CA. Creation of consistent burn wounds: a rat model. Archives of Plastic Surgery. 2014;41(4):317.
- Jalilimanesh M, Azhdari M, Mirjalili A, Mozaffari MA, Hekmatimoghaddam S. The comparison of clinical and histopathological effects of topical psyllium (Plantago ovata) powder and silver sulfadiazine on second-degree burn wound healing in rats. World Journal of Plastic Surgery. 2021;10(1):96.
- Hazrati M, Mehrabani D, Japoni A, Montasery H, Azarpira N, Hamidian-Shirazi AR, Tanideh N. Effect of honey on healing of Pseudomonas aeruginosa infected burn wounds in rat. Journal of Applied Animal Research. 2010;37(2):161-165.
- Fang J-Y, Lee W-R, Shen S-C, Huang Y-L. Effect of liposome encapsulation of tea catechins on their accumulation in basal cell carcinomas. Journal of Dermatological Science. 2006;42(2):101-109.
- Ma Q, Kuang Y, Hao X, Gu N. Preparation and characterization of tea polyphenols and vitamin E loaded nanoscale complex liposome. Journal of Nanoscience And Nanotechnology. 2009;9(2):1379-1383.
- Lu Q, Li D-C, Jiang J-G. Preparation of a tea polyphenol nanoliposome system and its physicochemical properties. Journal of Agricultural and Food Chemistry. 2011;59(24):13004-13011.
- Cui M-D, Pan Z-H, Pan L-Q. Danggui Buxue extract-loaded liposomes in thermosensitive gel enhance in vivo dermal wound healing via activation of the VEGF/PI3K/Akt and TGF-β/Smads signaling pathway. Evidence-Based Complementary and Alternative Medicine. 2017.
- Stevenson PC, Simmonds MS, Sampson J, Houghton PJ, Grice P. Wound healing activity of acylated iridoid glycosides from Scrophularia nodosa. Phytotherapy Research. 2002;16(1):33-35.
- Xie J, Yang Z, Zhou C, Zhu J, Lee RJ, Teng L. Nanotechnology for the delivery of phytochemicals in cancer therapy. Biotechnology Advances. 2016;34(4):343-353.
- Giri TK. Breaking the barrier of cancer through liposome loaded with phytochemicals. Current Drug Delivery. 2019;16(1):3-17.
- Takahashi M, Kitamoto D, Asikin Y, Takara K, Wada K. Liposomes encapsulating Aloe vera leaf gel extract significantly enhance proliferation and collagen synthesis in human skin cell lines. Journal of Oleo Science. 2009;58(12):643-650.
- Wu YC, Wu GX, Huang HH, Kuo SM. Liposome-encapsulated farnesol accelerated tissue repair in third-degree burns on a rat model. Burns. 2019;45(5):1139-1151.
)