Document Type : Original Article
1 Educated, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
2 Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
3 Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Objective- In diabetes impaired wound healing and other tissue abnormalities are considered as major concerns. The novel therapeutic options for treatment of wound in diabetic patients are urgently needed. The aim of the present study was to assess beneficial effects of chitosan nanocurcumin biofilm on healing of full thickness excisional wounds in diabetic rats.
Design- Experimental Study
Animals- Forty male diabetic Wistar rats
Procedures- The animals were randomized into four groups of ten animals each. Induction of diabetes was achieved using 60 mg/kg streptozotocin. In group I, 0.1 mL sterile saline 0.9% solution was added to the wounds with no dressing. In group II, the wounds were dressed with chitosan thin-film membranes. In group III, the wounds were treated with curcumin nanoparticles. In group IV, animals with were dressed with chitosan nanocurcumin biofilm.
Results- Planimetric, histological and quantitative morphometric studies and determination of hydroxyproline levels showed that there was significant difference between animals in group IV compared to other groups (p = 0.001).
Conclusion and clinical relevance- Chitosan nanocurcumin biofilm improved healing of diabetic wound in rats
- Venkatasubbu GD, Anusuya T. Investigation on Curcumin nanocomposite for wound dressing. International Journal of Biological Macromolecules, 2017; 98: 366-378.
- Almeida SA, Salomé GM, Dutra, RAA, Ferreira LM. Feelings of powerlessness in individuals with either venous or diabetic foot ulcers. Journal of Tissue Viability, 2014; 23(3): 109-114.
- Lee CH, Chang SH, Chen WJ, Hung KC, Lin YH, Liu SJ, Hsieh MJ, Pang JH, Juang JH. Augmentation of diabetic wound healing and enhancement of Collagen content using nanofibrous glucophage-loaded Collagen/PLGA scaffold membranes. Journal of Colloid and Interface Science, 2015; 439: 88–97
- Romero-Cerecero O, Zamilpa A, Díaz-García ER, Tortoriello J. Pharmacological effect of Ageratina pichinchensis on wound healing in diabetic rats and genotoxicity evaluation. Journal of Ethnopharmacology, 2014; 156: 222–227.
- Greenhalgh DG. Wound healing and diabetes mellitus. Clinics in Plastic Surgery, 2003; 30(1): 37-45.
- Kamar SS, Abdel-Kader DH, Rashed LA. Beneficial effect of Curcumin Nanoparticles-Hydrogel on excisional skin wound healing in type-I diabetic rat: Histological and immunohistochemical studies. Annals of Anatomy, 2019; 222: 94-102.
- Karri VV, Kuppusamy G, Talluri SV, Mannemala SS, Kollipara R, Wadhwani AD, Mulukutla S, Raju KR, Malayandi R. Curcumin loaded chitosan nanoparticles impregnated into collagen-alginate scaffolds for diabetic wound healing. International Journal of Biological Macromolecules, 2016; 93(Pt B): 1519-1529.
- Biagini G, Muzzarelli RAA, Giardiono R, Castaldini C. Biological material for wound healing. In: Brine CJ, Sanford PA, Zikakis JP, eds. Advances in chitin and chitosan. Elsevier, London, UK, 1992: 16–23.
- Muzzarelli RAA, Monica MB, Pugnaloni A, Biagini G. Biochemistry. Histology and clinical uses of chitins and chitosans in wound healing. In: Jolles P, Muzzarelli RAA, eds. Chitin and chitinases. Birkhauser Verlag, Basel, Switzerland; 1999: 251–264.
- Minami S, Okamoto Y, Tanioka S. Effects of chitosan on wound healing. In: Yalpani M, edr. Carbohydrates and carbohydrate polymers. ATL Press, Mount Prospect, IL, USA, 1992: 142-152.
- Minami S, Okamoto Y, Matsuhashi A, Sashiwa H, Saimoto Y, Shigemasa T, et al. Application of chitin and chitosan in small animals. In: Brine CJ, Sanford PA, Zikakis JP, edrs. Advances in chitin and chitosan. Elsevier Applied Science, Landon and New York, 1992: 70–78.
- Rouhollahi E, Moghadamtousi SZ, Hajiaghaalipour F, Zahedifard M, Tayeby F, Awang K, Abdulla MA, Mohamed Z. Curcuma purpurascens BI. rhizome accelerates rat excisional wound healing: involvement of Hsp70/Bax proteins, antioxidant defense, and angiogenesis activity. Drug Design, Development and Therapy, 2015; 9: 5805-5813.
- Pizzo P, Scapin C,Vitadello M, Florean C, Gorza L. Grp 94 acts as a mediator of curcumin-induced antioxidant defence in myogenic cells. Journal of Cellular and Molecular Medicine, 2010; 14(4): 970–981.
- Aggarwal B B, Harikumar KB. Potential therapeutic effects of curcumin: the anti-inflammatory agent against neurodegenerative cardiovascular, pulmonary, metabolic, autoimmune and neoplastic diseases. International Journal of Biochemistry & Cell Biology, 2009; 41(1): 40-59.
- Wang Y, Lu Z, Wu H, Lv F. Study on the antibiotic activity of microcapsule curcumin against foodborne pathogens. International Journal of Food Microbiology, 2009; 136(1): 71-74.
- Jovanovic SV, Boone CW, Steenken S, Trinoga M, Kaskey RB. How curcumin works preferentially with water-soluble antioxidants. Journal of the American Chemical Society, 2001; 123(13): 3064-3068.
- Tejada S, Manayi A, Daglia M, F Nabavi S, Sureda A, Hajheydari Z, Gortzi O, Pazoki-Toroudi H, M Nabavi S. Wound healing effects of curcumin: A short review. Current Pharmaceutical Biotechnology, 2016; 17(11): 1002-1007.
- Flora G, Gupta D, Tiwari A. Curcumin nanoparticles: a promising therapeutic advancement over native curcumin. Critical Reviews in Therapeutic Drug Carrier Systems, 2013; 30(4):331- 368.
- Kamel NA, Soliman AAF, Rozik NN, Abd-Elmessieh SL. Biophysical investigation of curcumin based nanocomposite for wound dressing application. Journal of Applied Pharmaceutical Science. 2018; 8(05): 35-44
- Zimmermann M. Ethical guidelines for investigations of experimental pain in conscious animals. Pain, 1983; 16: 109-110.
- Gonçalves RV, Novaes RD, Sarandy MM, Damasceno EM, da Matta SL, de Gouveia NM, Freitas MB, Espindola FS. 5α-Dihydrotestosterone enhances wound healing in diabetic rats. Life Sciences, 2016; 152: 67-75.
- Peters EJ, Lipsky BA. Diagnosis and management of infection in the diabetic foot. Medical Clinics of North America, 2013; 97(5): 911-946
- Okamoto Y, Sibazaki K, Minami S, Matsuhashi A, Tanioka S, Shigemasa Y. Evaluation of chitin and chitosan on open wound healing in dogs. Journal of Veterinary Medical Science, 1995; 57: 851–854.
- Okamoto Y, Tomita T, Minami S, Matsuhashi A, Kumazawa NH, Tanioka SI, Shigemasa Y. Effects of chitosan on experimental abscess with Stapylococcus aureus in dogs. Journal of Veterinary Medical Science, 1995; 57(4): 765-767.
- Mizuno K, Yamamura K, Yano K, Osada T, Saeki S, Takimoto N, Sakurai T, Nimura Y. Effect of chitosan film containing basic fibroblast growth factor on wound healing in genetically diabetic mice. Journal of Biomedical Materials Research Part A, 2003; 64(1): 177-181.
- Koji K. Clinical application of chitin artificial skin (Beschitin W). In: Brine CJ, Sanford PA, Zikakis JP, edrs. Advances in chitin and chitosan. Elsevier Applied Science, Landon and New York, 1992: 9-15.
- Azad AK, Sermsintham N, Chandrkrachang S, Stevens WF. Chitosan membrane as a wound-healing dressing: characterization and clinical application. Journal of Biomedical Materials Research Part B, 2004; 69: 216-222.
- Prasad R, Kumar D, Kant V, Tandan SK, Dinesh Kumar. Curcumin enhanced cutaneous wound healing by modulating cytokines and transforming growth factor in excision wound model in rats. International Journal of Current Microbiology and Applied Sciences,. 20176(7): 2263-2273.
- Bharti AC, Donaton N, Singh S, Aggarwal BB. Curcumin (diferuloylmethane) down-regulates the constitutive activation of nuclear factor-kappa B & I kappa B alpha kinase in human multiple myeloma cells, leading to suppression of proliferation and induction of apoptosis. Blood, 2003; 101(3): 1053–1062.
- Aggarwal BB. Signalling pathways of the TNF superfamily: a double-edged sword. Nature Reviews Immunology, 2003; 3: 745–756.
- Aggarwal BB, Shishodia S. Suppression of the nuclear factor-kappa B activation pathway by spice-derived phytochemicals: reasoning for seasoning. Annals of the New York Academy of Sciences, 2004; 1030: 434-441
- Jagetia GC, Rajanikant GK. Curcumin treatment enhances the repair and regeneration of wounds in mice exposed to hemibody gamma irradiation. Plastic and Reconstructive Surgery, 2005; 115(2): 515–528.
- Berridge MJ. Cell Stress, Inﬂammatory Responses and Cell Death. Cellular Signaling and Biology, 2014;2-14(1):1-30.
- Boyce ST, Lalley AL. Tissue engineering of skin and regenerative medicine for wound care. Burns and Trauma, 2018; 6: 4.
- Rodero M, Khosrotehrani K. Skin wound healing modulation by macrophages. International Journal of Clinical and Experimental Pathology, 2010; 3(7): 643–653.
- Mehrtash M, Mohammadi R, Hobbenaghi R. Effect of adipose derived nucleated cell fractions with chitosan biodegradable film on wound healing in rats. Wound Medicine, 2015; 10-11: 1-8
- Siamak Kazemi-Darabadi; Ghasem Akbari; Seyed-Hossein Jarolmasjed; Amir-Ali Shahbazfar. A Histopathologic Study of Effects of Olive Oil Plus Lime Water on Third-Degree Burn in Mouse Model. Iranian Journal of Veterinary Surgery, 2017; 12(1): 55-63.
- Elmore S. Apoptosis: a review of programmed cell death. Toxicologic Pathology, 2007; 35(4): 495-516.
- Wu YS, Chen SN. Apoptotic cell: linkage of inflammation and wound healing. Frontiers in Pharmacology, 2014; 5: 1.
- Rostami H, Mohammadi R, Asri-Rezaei S, Tehrani AA. Evaluation of application of chitosan/nano sodium selenite biodegradable film on full thickness excisional wound healing in rats. Iranian Journal of Veterinary Surgery, 2018; 13(1): 15-24.
- Shabrandi A, Azizi S, Hobbenaghi R, Ownagh A, Keshipour S. The healing effect of chitosan supported nano-CeO2 on experimental excisional wound infected with pseudomonas aeruginosa in rat. Iranian Journal of Veterinary Surgery, 2017; 12(2): 9-20.