The objective was to evaluate the ability of cinnamon nanoparticles (CNPs) in the healing of wounds with Pseudomonas aeruginosa (PAE) infection as well as HAMLET sensitization in rats. Fifty healthy male Wistar rats were used in this study. The rats were divided into 5 groups (n=10), randomly. In the Normal group, no infected wounds were treated with a sterile solution of saline 0.9% (0.1 ml). In the PAE group, the wounds with Pseudomonas aeruginosa infection were only treated with a sterile solution of saline 0.9% (0.1 ml). In the PAE-HMLT group, HAMLET (100 µg) was used to treat infected wounds. In the PAE-CNM group, 1 mg/ml CNPs (0.1 ml) was applied topically to treat PAE-infected wounds. In the PAE-HMLT-CNM group, HAMLET (100 µg) and 1 mg/ml CNPs (0.1 ml) were applied topically to treat PAE-infected wounds. Microbiological examination, planimetric and biochemical showed significant differences between rats in the PAE-HMT-CNM group in comparison with other groups (p < 0.05). In conclusion, CNPs could offer potential to pay more attention to this harmless and easily available agent to be topically applied in wounds with infection.