Objectives-The aims of this study were evaluation the effects of blood laser irradiation on hemodynamic parameters, the amount of anesthetic drugs in maintenance of anesthesia and duration of recovery. Animals- Fifteen mixed breed dogs Procedures- dogs (25.0 ± 3.6 kg) were divided into three groups (n=5). The group I was acted as control and received only cold laser. The group II was premedicated with neuroleptanalgesics including Midazolam (0.2 mg/kg/iv/bw) plus Fentanyl (0.05 mg/kg/i.v/bw), and induction of anesthesia was done by Propofol (5 mg /kg/i.v/bw), then dogs were intubated for maintenance of anesthesia by Isoflurane 1% In addition to neuroleptanalgesics combined with Isoflurane, group III received Trans-cutaneous Blood Laser Irradiation ( Trans-cut BLI) through the cephalic venous line by a portable laser probe designed by Canadian optic &laser center immediately after intubation.The changes in cardiac rhythm, hemodynamic parameters including heart rate, blood pressure, SpO2 and the level of blood cortisol were measured before and after the induction of anesthesia on 5, 10 ,15,45, and 60 minutes. Results- No complication from anesthesia and blood laser radiation was noticed, and despite of some variation observed in collective data but there was no significant difference between homodynamic parameters, blood cortisol level, SpO2 and the doses of Isoflurane. However all of dogs in group III had smooth recovery and attained full conscious after end of anesthesia. Conclusions and clinical relevance- This study showed that transcutaneous blood laser irradiation is a safe method without any side effect to add anesthetic procedure.There was no deterioration effect on hemodynamic parameters and consumption amount of Isoflurane.