Iranian Journal of Veterinary Surgery

Iranian Journal of Veterinary Surgery

Protective Effects of Ellagic Acid on Oxidative Stress and in vitro Fertilization in a Murine Model of Varicocele

Document Type : Original Article

Authors
Noor Noori-Abd 1
Gholamreza Najafi-Tazekand 2
Ali Shalizar-Jalali 2
Khalid Yassen Zakair Al-Zamily 3
Siamak Ghabeli-Zaherkandi 1
1 PhD Candidate, Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
2 Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
3 Kut Technical Institute, Middle Technical University, Baghdad, Iraq.
10.30500/ivsa.2024.484177.1420
Abstract
Varicocele is defined by abnormal tortuosity and pampiniform plexus veins dilation within the spermatic cord and is the most common surgically correctible cause of male infertility. This study aimed to evaluate the protective effect of ellagic acid (EL) on sperm parameters and in vitro fertilization (IVF) in a murine model of varicocele. In this experimental study, 50 mature male mice were randomly divided into five groups (n = 10), including control, varicocele, varicocele with a low dose of EL (25 mg/kg), varicocele with a medium dose of EL (50 mg/kg), and varicocele with a high dose of EL (100 mg/kg). After a 28-day treatment period, malondialdehyde, total anti-oxidant capacity (TAC), sperm parameters, and IVF were evaluated. In the varicocele group, TAC, and sperm count, motility, and viability, as well as zygotes, two-cell embryos, blastocysts, and hatched embryos were significantly reduced compared to the control group. Ellagic acid administration improved sperm parameters, fertilization rate, and embryo development. These findings suggest that EL owing to its anti-oxidant and free radicals scavenging abilities can inhibit the damaging effects of varicocele on fertility in mice.
Keywords
Ellagic acid
Varicocele
Sperm
In vitro fertilization
Mice

Subjects

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Iranian Journal of Veterinary Surgery
Volume 20, Issue 2 - Serial Number 43
October 2025
Pages 137-143

  • Receive Date 19 October 2024
  • Revise Date 21 December 2024
  • Accept Date 31 December 2024
  • First Publish Date 31 December 2024