Following surgical trauma to the peritoneum, bleeding and increased vascular permeability occur, accompanied by the leakage of fibrinogen-rich fluids from the damaged surfaces. Peritoneal adhesions are observed in 60 to 93 percent of patients after abdominal surgeries and are one of the most common causes of long-term complications following such procedures. this study aimed to examine the combined effect of platelet-rich plasma (PRP) gel and the drug dexamethasone in preventing intra-abdominal adhesions following laparotomy in rats. In this research, 35 adult male Wistar rats, weighing between 250 and 300 grams, were selected. Fifteen rats were used to prepare PRP, and blood collection was performed after anesthesia with ketamine/xylazine. The remaining 20 rats were divided into four groups: the control group (C), the PRP-treated group (P), the dexamethasone-treated group (D), and the group receiving the combination of PRP and dexamethasone (PD). To induce adhesions, sterile tampon friction was applied to the serosal surface of the poorly vascularized descending colon area, and additional friction was created using a surgical blade on the peritoneal surface of the colon. The relevant treatments were then injected into each group. Results showed no significant differences in fibrinogen levels and leukogram between the groups (p > 0.05); however, macroscopic examination indicated a reduction in adhesions in the PRP group compared to the other groups (p = 0.001). According to the results of this research, it is concluded that the addition of dexamethasone to platelet-rich plasma will not have a significant effect on reducing the state of adhesion following abdominal surgery in rats.