This study was conducted to evaluate the healing effects of different methods of using ozone in third-degree skin burns in the rat experimental model. To carry out this study, 45 adult male rats with an average weight of 250-300 g were divided into 5 groups of 9, including the first group of treatment with ozonated water, the second group of ozonated oil, the third group of ozonated gas, and the fourth group of systemic ozone gas. The fifth group was used as a control group. To create a burn, the innovative device, whose accuracy has been confirmed in previous studies, was used with a 2 × 3 cm probe with a temperature of 95 °C and 10 seconds of contact with the skin. Wound management was performed every other day for up to 14 days, and daily photographs were taken of the surface of the wounds. On the 4th, 8th, and 14th day, samples were taken for histopathologic examination, the amount of tissue hydroxyproline was also examined on the 14th day of the study. The statistical data obtained were analyzed with SPSS software and one-way analysis of variance statistical tests, values less than 5% were considered as significant levels. Morphologic examination of the wound healing process and histopathologic observations showed that the use of ozonated water and ozonated oil significantly accelerates (p < 0.05) the wound healing process compared to other groups and reduces the infiltration of inflammatory cells, improves angiogenesis and fibroplasia in the wound site. It was also found that the amount of tissue hydroxyproline in the ozonated oil group was lower than in the other studied groups (p < 0.05). According to the findings of the current study, it seems that the use of ozone in liquid phases, i.e. ozonated water and ozonated oil, has a better effect on the wound healing process than ozone in gaseous form.