Iranian Journal of Veterinary Surgery

Iranian Journal of Veterinary Surgery

The Effect of β-Cryptoxanthin on Testicular Ischemia-Reperfusion Injury in a Rat Model: Evidence from Testicular Histology

Document Type : Original Article

Authors
Kianoush Mohammadnejad 1
Rahim Mohammadi 1
Ali Soleimanzadeh 2
Ali Shalizar-Jalali 3
Farshid Sarrafzadeh-Rezaei 1
1 Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
2 Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
3 Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
10.30500/ivsa.2024.457134.1405
Abstract
Testicular torsion and detorsion are significant clinical issues for infertile man. Torsion of the spermatic cord is an emergency condition resulting from rotation of the testis and epididymis around the axis of the spermatic cord. A rat testis model was used to assess effects of β-cryptoxanthin on ischemia-reperfusion injury. Twenty healthy male Wistar rats were included and randomized into four investigational groups (n = 5): Group SHAM: In this group, midline incision of the scrotum was performed and the testicles were taken out for 2 hours with a 720-degree rotation. Group ISCHEMIA: In this group, midline incision of the scrotum was performed and the testicles were taken out and undergone ischemia for 2 hours with a 720-degree rotation. Group IS/REP/Oil: In this group, a midline scrotum cut was performed and the testicles were taken out and ischemia was created for 2 hours with a 720-degree rotation and at the end of ischemia 100 µl of corn oil (β-cryptoxanthin solvent) was injected intraperitoneally. Group IS/REP/CRPTXNTN 2.5: The same as group IS/REP/Oil as well as intraperitoneal administration of 100 µl of β-cryptoxanthin (2.5 µg/kg) at the end of ischemia. In all groups, the testes were returned back to scrotum and after 60 days were dissected out and removed for histopathological analyses. β-cryptoxanthin at the dose of 2.5 µg/kg significantly improved histologic indices compared to other treatment groups (p < 0.05). β-cryptoxanthin could be helpful in minimizing ischemia-reperfusion injury in testicular tissue exposed to ischemia.
Keywords
β-cryptoxanthin
Ischemia-reperfusion
Torsion
Testis

Subjects

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Iranian Journal of Veterinary Surgery
Volume 20, Issue 2 - Serial Number 43
October 2025
Pages 110-115

  • Receive Date 12 May 2024
  • Revise Date 29 July 2024
  • Accept Date 12 August 2024
  • First Publish Date 12 August 2024