Iranian Journal of Veterinary Surgery

Iranian Journal of Veterinary Surgery

The Protective Roles of Mito-TEMPO on Testicular Ischemia‑Reperfusion Injury Based on Biochemical and Histopathological Evidences in Mice

Document Type : Original Article

Authors
Zohreh Mostahsan 1
Saeed Azizi 1
Ali Soleimanzadeh 2
Ali Shalizar-Jalali 3
1 Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
2 Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
3 Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
10.30500/ivsa.2024.448516.1394
Abstract
Accumulation of reactive oxygen species during testicular torsion causes oxidative stress, which in turn causes ischemia-reperfusion (I/R) injury to the testis. In testicular torsion/detorsion (T/D) in male mice, the purpose of this study was to investigate the influence of Mito-TEMPO (MT) on I/R injury. Forty-two male mice were divided into seven groups, including a control group and six treatment groups (360° T/D, 720° T/D, 360° T/D + 0.70 mg/kg MT, 360° T/D + 1 mg/kg MT, 720° T/D + 0.70 mg/kg MT and 720° T/D + 1 mg/kg MT). After inducing testicular torsion, oxidative enzymes, and testicular histopathology were evaluated. The results showed that 720° T/D resulted in increased testicular malondialdehyde levels and histological damage, along with reduced activities of catalase, superoxide dismutase, and glutathione peroxidase. Treatment with MT reduced tissue malondialdehyde levels and increased the activities of catalase, superoxide dismutase, and glutathione peroxidase. These findings suggest that MT administration protects against acute testicular T/D injury in mice.
Keywords
Mito-TEMPO
Ischemia‒reperfusion injury
Oxidative stress
Testicular histopathology

Subjects

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  • Receive Date 14 March 2024
  • Revise Date 11 May 2024
  • Accept Date 18 May 2024
  • First Publish Date 18 May 2024