Iranian Journal of Veterinary Surgery

Iranian Journal of Veterinary Surgery

Nano-Methoxatin Improves Ischemia-Reperfusion Injury in Torsion-Detorsion Model of Ovary

Document Type : Original Article

Authors
Kave Koorehpaz 1
Alireza Najafpour 1
Rahim Mohammadi 2
1 Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran.
2 Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
10.30500/ivsa.2023.378615.1329
Abstract
Using a rat ovary model, protective effects of nano-methoxatin (NMXTN) were studied on ischemia-reperfusion injury. Following randomization of forty-eight healthy female Wistar rats ~250 g, the animals were subjected to eight experimental groups (n = 6): Group SHAM: Only laparotomy was performed. Group IS: Only a 3-hour ischemia was performed. Group IS/Oil: Only a 3-hour ischemia was performed and 50 µl soybean oil alone (Solvent of MXTN) was administered 30 min before cessation of ischemia. Group IS/REP: The procedure included a 3-hour ischemia followed by a 3-hour reperfusion and 50 µl soybean oil alone was administered 30 min before cessation of ischemia. Group IS/MXTN: The procedure included a 3-hour ischemia only and 50 µl (0.3 mmol/l/IP) of MXTN 30 min before cessation of ischemia. Group IS/NMXTN: The procedure included a 3-hour ischemia only and 50 µl (0. 3 mmol/l/IP) of NMXTN 30 min before cessation of ischemia. Group IS/REP/MXTN: The procedure included a 3-hour ischemia, a 3-hour reperfusion and 20 µl (0.3 mmol/l) of MXTN 30 min before cessation of ischemia. Group IS/REP/NMXTN: The procedure included a 3-hour ischemia, a 3-hour reperfusion and 20 µl (0.3 mmol/l) of NMXTN 30 min before cessation of ischemia. Significantly amended development of ischemia/reperfusion tissue injury was observed in animals treated with NMXTN compared to those of other groups (p = 0.001). Mean values of biochemical indices were significantly higher than those observed for other groups (p = 0.001). Significantly lower values of MDA were observed in IS/REP/NMXTN animals compared to those of other groups (p = 0.001). Where ovarian tissue is exposed to ischemia intraperitoneal administration of NMXTN could bear clinical benefits in diminishing ischemia-reperfusion injury.
Keywords
Nano-methoxatin
Ischemia-reperfusion
Intraperitoneal
Ovary

Subjects

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Iranian Journal of Veterinary Surgery
Volume 18, Issue 2 - Serial Number 39
October 2023
Pages 106-112

  • Receive Date 28 December 2022
  • Revise Date 04 March 2023
  • Accept Date 15 March 2023
  • First Publish Date 15 March 2023