Iranian Veterinary Surgery Association (IVSA) Iranian Journal of Veterinary Surgery 2008-3033 10 2 2015 12 01 Effect of Local Administration of Laminin and Fibronectin with Chitosan Conduit on Peripheral Nerve Regeneration: A Rat Sciatic Nerve Transection Model 39 47 12281 EN Yusef Nasiri Young Researchers and Elite Club, Urmia Branch, Islamic Azad University, Urmia, Iran Rahim Mohammadi Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University 0000-0002-2104-9829 Journal Article 2014 07 23 Objective-Effect of local administration of laminin and fibronectin on nerve regeneration was assessed.<br /> Design- Experimental study.<br /> Animal- Sixty male Wistar rats.<br /> Procedures- The animals were divided into four experimental groups (n=15), randomly: In transected group left sciatic nerve was transected and stumps were fixed in adjacent muscle. In treatment group (CHIT/LF) the defect was bridged using a chitosan conduit filled with 10 µL mixture at a concentration of 1 mg/ml laminin and 1 mg/ml fibronectin in a 1:1 volumetric addition. In CHIT group the conduit was filled with phosphate-buffered saline. In normal control group sciatic nerve was exposed and manipulated. Each group was subdivided into three subgroups of five animals each and nerve fibers were studied in a 12-week period.<br /> Results- Behavioral, functional, gastrocnemius muscle mass findings and morphometric indices confirmed faster recovery of regenerated axons in CHIT/LF than in CHIT group (p<0.05). Immunohistochemical reactions to S-100 in CHIT/LF were more positive than that in CHIT group.<br /> Conclusion and Clinical Relevance- Laminin-fibronectin improved functional recovery and morphometric indices of sciatic nerve. They could be considered as an effective treatment for peripheral nerve repair in practice. https://www.ivsajournals.com/article_12281_fb51aea2099d8fbdfe50a5cd69ff8e50.pdf