TY - JOUR ID - 57950 TI - In Vivo Assessment of Anti-Tumor Activity of Methyl 2'-Methyl-1,3-dioxo-1,1',2',3,5',6',7',7a'-octahydrospiro[indene-2,3'-pyrrolizidine]-2' carboxylate (6) on 7,12 dimethylbenz(a) Anthracene-Induced Mammary Tumors in Rat JO - Iranian Journal of Veterinary Surgery JA - IVSA LA - en SN - 2008-3033 AU - Zarei, Leila AU - Kouhkan, Mehri AU - Bolouri, Ali AU - Mohammadi, Rahim AD - Razi Herbal Medicines Research Center, Lorestan University of medical Sciences, Khorramabad, Iran. AD - Department of Medical Chemistry, School of Pharmacy, Urmia University of Medical Sciences, Urmia, Iran AD - Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University Y1 - 2017 PY - 2017 VL - 12 IS - 2 SP - 53 EP - 62 KW - Breast carcinogenesis KW - 7-12-Dimethylbenz(a)anthracene KW - Indene-2-3'-pyrrolizidine KW - mammary tumors DO - 10.22034/ivsa.2018.111321.1131 N2 - Objective- Breast cancer is one of the most serious problems in oncology. Alkaloids pyrrolizidine are widely found in nature and exhibited versatile biological activities. The aim of the present study was to assess anti-tumor activity of methyl 1,3-dioxo-1,1',2',3,5',6',7',7a'-octahydrospiro[indene-2,3'-pyrrolizidine]-2'-carboxylate (6) on 7,12 dimethylbenz(a) anthracene (DMBA)-induced mammary tumors in rat. Design- Experimental study.Animals- Twenty-one female Wistar atsProcedures- 7-Week-old female Wistar rats (180 g body weight) were randomized into three groups of seven animals each. DMBA-induced mammary tumors was achived using DMBA dissolved in 1ml of vehicle (0.5 ml of DMSO plus 0.5 ml of saline) and injected by subcutaneous injection beneath the mammary gland on either side. Tumor yield and size were stabilized after 90 days with the initiation of DMBA. Healthy intact animals (NC) were considered as a normal control feeding on pellets and tap water. In group DMBA, the tumor was induced by DMBA. In group DMBA/7d the animals with tumor received 100 µL 6 dissolved in DMSO (0.25 µM) solution intraperitoneally for one week. Synthesis of 6 was accomplished and the structure of new product was assigned by their 1H/13C NMR, IR, CH-COSY and mass spectral data as well as the elemental analysis. Results- The overall tumor analysis showed that 6 treatment significantly inhibited the breast tumor incidence, tumor multiplicity, and tumor size in the DMBA-initiated rat model. 6 treatment significantly inhibited the total volume of tumors per rat. Conclusion and Clinical Relevance-The findings of the present study showed that the 6 treatment was significantly effective in reduction of tumors versus the cancer controls. UR - https://www.ivsajournals.com/article_57950.html L1 - https://www.ivsajournals.com/article_57950_77617eb06832defa2a33ae58af9c716e.pdf ER -