TY - JOUR ID - 12281 TI - Effect of Local Administration of Laminin and Fibronectin with Chitosan Conduit on Peripheral Nerve Regeneration: A Rat Sciatic Nerve Transection Model JO - Iranian Journal of Veterinary Surgery JA - IVSA LA - en SN - 2008-3033 AU - Nasiri, Yusef AU - Mohammadi, Rahim AD - Young Researchers and Elite Club, Urmia Branch, Islamic Azad University, Urmia, Iran AD - Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University Y1 - 2015 PY - 2015 VL - 10 IS - 2 SP - 39 EP - 47 KW - Nerve repair KW - Sciatic KW - Fibronectin KW - Laminin KW - Local KW - Rat DO - N2 - Objective-Effect of local administration of laminin and fibronectin on nerve regeneration was assessed. Design- Experimental study. Animal- Sixty male Wistar rats. Procedures- The animals were divided into four experimental groups (n=15), randomly: In transected group left sciatic nerve was transected and stumps were fixed in adjacent muscle. In treatment group (CHIT/LF) the defect was bridged using a chitosan conduit filled with 10 µL mixture at a concentration of 1 mg/ml laminin and 1 mg/ml fibronectin in a 1:1 volumetric addition. In CHIT group the conduit was filled with phosphate-buffered saline. In normal control group sciatic nerve was exposed and manipulated. Each group was subdivided into three subgroups of five animals each and nerve fibers were studied in a 12-week period. Results- Behavioral, functional, gastrocnemius muscle mass findings and morphometric indices confirmed faster recovery of regenerated axons in CHIT/LF than in CHIT group (p<0.05). Immunohistochemical reactions to S-100 in CHIT/LF were more positive than that in CHIT group. Conclusion and Clinical Relevance- Laminin-fibronectin improved functional recovery and morphometric indices of sciatic nerve. They could be considered as an effective treatment for peripheral nerve repair in practice. UR - https://www.ivsajournals.com/article_12281.html L1 - https://www.ivsajournals.com/article_12281_c8b4156d9959801ba2bb04564a00401b.pdf ER -