Objectives- To evaluate the efficacy of platelet rich- plasma (PRP) and plasma rich-growth factor (PRGF®) on the wound healing on distal aspect of horses limbs. Design- Experimental study Animals - Four mixed- breed adult horses, 13 to 21 years of age and weighting 381 ±85 kg Procedures- The Limbs of each horse were treated with PRP, PRGF®, Silver Sulfa diazin and one limb received no treatment (only washing with normal saline). In this manner, each horse served as its own control. Latin square was selected as wound distribution model. Results- There were significant differences between epithelialization, percent of contraction, granular tissue formation, granular tissue surface situation, wound exudation, inflammation severity and angiogenesis in PRP and PRGF® groups, but there were no significant differences between wound surface area, inflammation expansion and fibroplasia. Conclusion and Clinical Relevance- Due to special trait of healing at distal aspect of horse limb which exuberant granulation tissue can cause dilation, use of PRP and PRGF® to accelerate wound healing at this region are not recommendable.