Objectives- To evaluate radiographic changes and macroscopic findings in handsewn laparoscopic gastrojejunostomy in dogs. Study Design-Elective experimental study Animals-Eighteen mix-breed healthy male and female dogs. Methods-Animals were divided in two equal groups randomly. Two layers handsewn laparoscopic side to side gastrojejunostomy was performed in the control group and one layer in the treatment group. Cases were monitored for four weeks. Contrast radiographs were taken in the first and last week of the study. Cases were sacrificed after four weeks and macroscopic findings were evaluated at the anastomotic site and it’s diameter was measured. Results-There was no evidence of stricture, stenosis or organ displacement radiographically. No infection, stricture, fistulae formation, abscess, ischemia or gross granulation tissue was seen, but several adhesions were found in the abdominal cavity macroscopically. The most frequent adhesion was between omentum and the anastomotic site which was reported in all cases. There were no statistically significant difference between the anastomotic diameter in both groups (P>0.05). Conclusion and clinical relevance- One layer laparoscopic handsewn gastrojejunostomy is a safe and feasible technique without serious complication with no statistically significant difference with the conventional two-layer technique.