Objectives-To evaluate the clinical safety and feasibility of one layer suture pattern technique in gastrojejunostomy in dog in comparison to two layer suture pattern technique. Study Design-Elective experimental study Animals-Sixteen mix-breed healthy male and female dogs Methods-Sixteen healthy male and female dogs were randomly divided in two groups. In the control group the two layer side to side laparoscopic gastrojejunostomy were performed. In the case group the one layer anastomosis between stomach and jejunum were done. Clinical parameters include body temperature, heart rate, respiratory rate, CRT, mean surgical time, blood loss, surgical complications, CBC, total protein, albumin and weight loss were evaluated. Results-There were no significant differences between body temperature, heart rate, respiratory rate, CRT, CBC, total protein and albumin in both groups. There was significance difference between mean surgical time and blood loss and in two groups. Conclusion and Clinical Relevance- Laparoscopic gastrojejunostomy with one layer closure technique based on clinical data revealed several advantages include feasibility, safety and less time consuming in comparison to two layer suture pattern technique.