Document Type: Original Article

Authors

1 Developmental Biology and Regenerative Medicine Research Nucleus,

2 Graduated Student, and Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran,

3 Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran,

4 Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, Iran.

Abstract

Objective-  In the present study, we investigated the isolation protocol, population doubling time (PDT) and the expression of a pluripotential gene by RT-PCR analysis of early transcription factor Nanog in caprine umbilical cord (CUC) Wharton's jelly mesenchymal cells (WJMCs).
Design- Experimental in vitro study.  
Animals- Four mix breed goat.  
Procedures- CUCs were collected from abattoir pregnant uteri and their Wharton's jellies (WJs) were cut into 2×2 mm2  segments for explanting. 8-10 segments were explanted into each 35 mm culture dish. WJ explants were removed 5 days after plating and the remaining adherent cells were cultured for another 5 days. Isolated cells were histochemically assayed for the presence of alkaline phosphatase (AP) activity. RT-PCR was used to assess the presence of Nanog mRNA. Besides, in this study the growth kinetic was evaluated for the isolated cells.
Results-  CUC isolated cells displayed spindle-form and small round-shape with high nucleus. Confluent cells formed colonies that indicated AP activity. Initial seeding concentration of 2 × 104  CUC-WJMCs resulted in shorter PDT compared to fetal fibroblasts (46.57 vs. 54.29 hours, respectively). Expression of Nanog was undetectable in 9th  passage of CUC-WJMCs.
Conclusion and Clinical Relevance- CUC contains an easily obtainable source of mesenchymal cells which exhibit stem cells  properties but probably these cells are not pluripotent.

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