Cancer is an uncontrolled growth of abnormal cells. It is found in animals as well as humans. There are many different types of cancer that are found in animals, symptoms are often similar to those in people. An 8-year-old male donkey was referred to the Veterinary Teaching Hospital and examined for cylinder-like dense mass that was found on the top of the cervical region and extended from the dorsal part of the base of the ears to withers. Radiographic evaluation of the cervical region was demonstrated that the mass was well-demarcated soft tissue opacity in dorsal cervical vertebrae. Ultrasonographic experimentation display a well circumscribe hyperechoic triangular shape mass with fat echogenicity in the sagittal plane without any vascularization. Surgical exploration of the mass indicated fusiform to cylindrical, well-demarcated, and soft tissue in yellow color. The microscopic finding showed that the tumor cells were identical to those in normal fat tissue and massive vacuoles exchange the cytoplasm with peripheralization of the nuclei. Necrosis and inflammation were not observed, while, dystrophic calcification and fibrosis were detected. Based on the aforesaid macro-, microscopic and histopathologic findings, Lipoma was recognized. Nevertheless, no treatment was performed for this case due to the large volume of the mass.