Honey, Curcuma, Ginger, and Nigella sativa are known for their antioxidant, immune stimulation properties, and wound healing effects. This study was conducted to determine the effect of these compounds and their mixture on the bone healing of the radial bone defect model in rats. 110 radial bone defects in 55 healthy male rats were randomly divided into 11 groups (10 rats per group). Group 1-5 received honey, Curcuma, Ginger, and Nigella sativa orally by gavage, and group 6-10 received the same materials locally by rubbing them on the sutured skin. The bone repair effects of these materials were evaluated by histopathology and biomechanical examinations. Using honey, Curcuma, Ginger, and Nigella sativa exhibited a beneficial effect to promote bone healing in this study. The biomechanical and histopathology evaluation showed that the honey (oral), a mixture of honey and Curcuma (oral), and a mixture of honey and Nigella sativa (topical) groups, exhibited significantly better result at 52nd postoperative day compared to the other groups as well as the control group.