Document Type : Original Article
Graduate of Veterinary Medicine, Shahrekord Branch Islamic Azad University, Shahrekord, Iran.
Department of Pathobiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Department of Orthopedic Surgery, Rasoul Akram Hospital, Iran University of Medical Sciences, Tehran, Iran.
Objective- Veterinary and human orthopedics is widely applied to stimulate fracture healing, accelerate junction connection, and repair bone defects. In this regard, several studies have been conducted on selecting the best material for bone repair. This study aimed to evaluate the effect of aqueous extract of Elaeagnus angustifolia fruit on the process of bone repair in a rabbit animal model.
Design- Experimental Study
Animals- For this purpose, 12 rabbits were obtained.
Procedures- Rabbits randomly divided into 3 groups. The control group, in which a two-sided hole was created in the femur and did not receive the treatment. The second group underwent surgery and received an aqueous extract of Elaeagnus angustifolia fruit at a dose of 250 mg/kg, and the third group received 500 mg/kg intraperitoneal. On weeks 2 and 4 following the bone defect, a histopathologic sample was taken from the site. The bone tissue samples were stained with hematoxylin and eosin.
Results-According to histopathologic results, the best and weakest performances occurred in the group treated with 500 mg/kg of extract and in the control group, respectively. This group had a significant difference with other groups in terms of osteoplasia (p < 0.05).
Conclusion and Clinical Relevance- In general, the results showed that the aqueous extract of Elaeagnus angustifolia can play an important role in the healing of bone fractures.
- Loi L, Córdova AJ, Pajarinen TH, Lin ZSB, Goodman SB. Inflammation, fracture and bone repair. Repair, 2016; 86: 119–130.
- Tu N, Lie D, Wan C. K. Osteoporosis: a review of treatment options. Journal of Pharmacy and Therapeutic, 2018; 3: 92–104.
- Scott H, McLaughlin R. Feline orthopedics. 1st ed. CRC, 2006; 302-303.
- Kusova RD, Kazakov A, Luk’yanchikov MS. Phenolic compounds from fruit of Elaeagnus angustifolia. Chemistry of Natural Compound, 1998; 24: 392-393.
- Hokmabad VR, Davaran S, Aghazadeh M, Alizadeh E, Salehi R. Effect of incorporating Elaeagnus angustifolia extract in PCL-PEG-PCL nanofibers for bone tissue engineering. Frontiers of Chemical Science and Engineering, 2019; 13: 108-119.
- Hedin H, Hjorth K, Larsson S, Nilsson S. Radiological outcome after external fixation of 97 femoral shaft fractures in children. Injury, 2003; 34: 287-292.
- Panahi Y, Alishiri GH, Bayat N, Hosseini, SM, Sahebkar, A. Efficacy of Elaeagnus angustifolia extract in the treatment of knee osteoarthritis: a randomized controlled trial. Excli Journal, 2016; 15: 203.
- Natanzi MM, Pasalar P, Kamalinejad M, Dehpour AR, Tavangar, SM. Effect of aqueous extract of Elaeagnus angustifolia fruit on experimental cutaneous wound healing in rats. Acta Medica Iranica, 2012; 50: 589-596.
- Wang Q, Ruan X, Huang JH, Xu NY, Yan QC. Intra-specific genetic relationship analyses of Elaeagnus angustifolia based on RP-HPLC biochemical markers. Journal of Zhejiang University-Science, 2006; 7: 272-278.
- Ahmadiani A, Hosseiny J, Semnanian S, Javan M, Saeedi, F. Antinociceptive and anti-inflammatory effects of Elaeagnus angustifolia fruit extract. Journal of Ethnopharmacology, 2000; 72: 287-292.
- Hosseinzadeh H, Ramezani M, Namjo N. Muscle relaxant activity of Elaeagnus angustifolia L. fruit seeds in mice. Journal of Ethnopharmacology, 2003; 84: 275-278.
- Rabiei K, Ghobadifar A, Ebrahimzadeh MA, Saeedi M, Mobini M. Effects of ginger and Elaeagnus angustifolia extracts in symptomatic knee osteoarthritis. Journal of Research in Medical Science, 2015; 17: 1-6.
- Nikniaz Z, Mahdavi R, Ostadrahimi A, Ebrahimi A, Nikniaz L. Effects of Elaeagnus angustifolia L. powder supplementation on serum total antioxidant capacity and malondialdehyde levels in females with knee osteoarthritis. Journal of Herb Medicine, 2015; 5: 177-183.
- Estai MA, Soelaiman IN,Shuid AN. Histological changes in the fracture callus following the administration of water extract of Piper sarmentosum (Daun Kadok) in estrogen-deficient rats. Iranian Journal of Medical Science, 2011; 36(4): 281–288.