A nine years old New Zealand white female diabetic rabbit was presented to the Department of Veterinary Clinical Complex OPD, Kolkata, India with a history of chronic weight loss along with inappetence, polyuria, and hematuria from the last 10 days. Feeding history revealed that the animal was maintained with a mixed diet that is rich in calcium (Ca). Manual examination revealed moderate pain near the caudal region and a hard mass was felt near the bladder region and lateral radiographs revealed the presence of radio-opaque cystolith. Cystotomy was performed and the rabbit was recovered uneventfully. The analysis of the stone revealed calcium carbonate as the major content. The present article describes successful surgical management of urolith in a rabbit and confers ample evidence that a high calcium diet, peculiar Ca metabolism along with more carbohydrate predisposing to diabetes may setup urolithiasis. The present study also emphasizes the fact that diminished Na+-K+-ATPase activity in diabetes can play a pivotal role in the pathogenesis of peripheral vascular and neural complications leading to the development of bladder dysfunction, hence diminished urine flow in diabetic rabbits results in the development of nidus formation and thereby further accumulation of calcium salts in and around nidus leads to the formation of urolithiasis.