Document Type : Original Article

Authors

1 Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran.

2 Department of Anatomical Sciences, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.

3 Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.

Abstract

The aim of the present study was to assess the wound-healing activity of extract of Allium stipitatum. Thirty-six male Wistar rats were used in this study. The rats weighing approximately 160-180 g and seven weeks of age were randomized into three groups of 12 rats each: Control surgery group (Control) including the creation of wounds and no treatment, base formulation groups positive (POS) with the creation of wounds and application of base formulation ointment, treatment group 1 (T1) with 2 g of powder extract of the plant material in the ointment. A wound was induced by an excisional based wound model in male rats. The mature green leaves of Allium stipitatum were collected and authenticated. Extractions of dried leaves were carried out. For wound-healing activity, the extracts were applied topically in the form of ointment and compared to control groups. The healing of the wound was assessed based on the wound area, histomorphometry, and hydroxyproline estimation studies. Reduction in the wound area and hydroxyproline contents indicated that there was a significant difference (p = 0.001) between group T1 and other groups. Quantitative histological studies and mean rank of the qualitative studies demonstrated that there was a significant difference between group T1 and other groups (p = 0.001). The extract of Allium stipitatum leaves enhanced wound-healing activity significantly in both the wound models studied. Enhanced wound contraction, decreased epithelialization time, increased hydroxyproline content, improved histological characteristics studies suggested Allium stipitatum leaves extract might have therapeutic benefits in wound healing.

Keywords

Main Subjects

  1. Shedoeva A, Leavesley D, Upton Z, Fan C. Wound healing and the use of medicinal plants. Evidence Based Complementary and Alternative Medicine, 2019.
  2. Ghahary A, Ghaffari A. Role of keratinocyte–fibroblast cross-talk in development of hypertrophic scar. Wound Repair and Regeneration, 2007; 15(1): 46–53.
  3. Deshmukh PT, Gupta VB. Embelin accelerates cutaneous wound healing in diabetic rats. Journal of Asian Natural Products Research, 2013; 15(2): 158-165.
  4. Eming SA, Martin P, Tomic-Canic M. Wound repair and regeneration: mechanisms, signaling, and translation. Science Translational Medicine, 2014; 6(265): 265.
  5. Asgarpanah, J, Ghanizadeh, B. Pharmacologic and medicinal properties of Allium hirtifolium Boiss. African Journal of Pharmacy and Pharmacology, 2012; 6(25): 1809–1814.
  6. Krejčová P, Kučerová P, Stafford GI, Jäger AK, Kubec R. Antiinflammatory and neurological activity of pyrithione and related sulfur-containing pyridine N-oxides from Persian shallot (Allium stipitatum). Journal of Ethnopharmacology, 2014; 154(1): 176–182.
  7. Bay-Smidt MGK, Jäger AK, Krydsfeldt K, Meerow AW, Stafford GI, Van Staden J, Rønsted N. Phylogenetic selection of target species in Amaryllidaceae tribe Haemantheae for acetylcholinesterase inhibition and affinity to the serotonin reuptake transport protein. South African Journal of Botany, 2011; 77(1): 175–183.
  8. Djeridane A, Yoush M, Nadjemi B, Boutassouna D, Stockor P, Vidal N. Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds. Food Chemistry, 2006; 97(4): 654-660.
  9. Yasin H, Mahmud S, Rizwani GH, Perveen R, Abrar H, Fatima K. Effects of aqueous leaves extract of Holoptelea integrifolia (Roxb) planch on liver and kidney histopathology of albino rats. Pakistan Journal of Pharmaceutical Sciences, 2019; 32(2): 569-573.
  10.  Qiu Z, Kwon AH, Kamiyama Y. Effects of plasma fibronectin on the healing of full-thickness skin wounds in streptozotocin-induced diabetic rats. Journal of Surgical Research, 2007; 138(1): 64-70.
  11.  Singer AJ, Clark RA. Cutaneous wound healing. New England Journal of Medicine, 1999; 341(10): 738–746.
  12.  Rodrigues M, Kosaric N, Bonham CA, Gurtner GC. Wound healing: A cellular perspective. Physiological Reviews, 2019; 99(1): 665–706.
  13.  Sorg H, Tilkorn DJ, Hager S, Hauser J, Mirastschijski U. Skin wound healing: an update on the current knowledge and concepts. European Surgical Research, 2017; 58(1-2): 81-94.
  14.  Wang XJ, Han G, Owens P, Siddiqui Y, Li AG. Role of TGF beta-mediated inflammation in cutaneous wound healing. Journal of Investigative Dermatology Symposium Proceedings, 2006; 11(1): 112–117.
  15.  Trengove NJ, Bielefeldt-Ohmann H, Stacey MC. Mitogenic activity and cytokine levels in non-healing and healing chronic leg ulcers. Wound Repair and Regeneration, 2000; 8: 13–25.
  16.  Wetzler C, Kampfer H, Stallmeyer B, Pfeilschifter J, Frank S. Large and sustained induction of chemokines during impaired wound healing in the genetically diabetic mouse: Prolonged persistence of neutrophils and macrophages during the late phase of repair. Journal of Investigative Dermatology, 2000; 115(2): 245–253.
  17.  McCarty SM, Percival SL. Proteases and Delayed Wound Healing. Advances in Wound Care, 2013; 2(8): 438–447.
  18.  Rodrigues M, Kosaric N, Bonham CA, Gurtner GC. Wound Healing: A Cellular Perspective. Physiological Reviews, 2019; 99(1): 665–706.
  19.  Dreifke MB, Jayasuriya AA, Jayasuriya AC. Current wound healing procedures and potential care. Materials Science and Engineering: C, 2015; 48: 651–662.
  20.  Maione AG, Smith A, Kashpur O, Yanez V, Knight E, Mooney DJ, Veves A, Tomic-Canic M, Garlick JA. Altered ECM deposition by diabetic foot ulcer-derived fibroblasts implicates fibronectin in chronic wound repair. Wound Repair and Regeneration, 2016; 24(4): 630–643.
  21.  Pastar I, Stojadinovic O, Yin NC, Ramirez H, Nusbaum AG, Sawaya A, Patel SB, Khalid L, Isseroff RR, Tomic-Canic M. Epithelialization in Wound Healing: A Comprehensive Review. Advances in Wound Care, 2014; 3(7): 445–464.
  22.  André-Lévigne D, Modarressi A, Pepper MS, Pittet-Cuénod B. Reactive oxygen species and NOX enzymes are emerging as key players in cutaneous wound repair. International Journal of Molecular Sciences, 2017; 18(10): 2149.
  23.  Patil PS, Evancho-Chapman MM, Li H, Huang H, George RL, Shriver LP, Leipzig ND. Fluorinated methacrylamide chitosan hydrogel dressings enhance healing in an acute porcine wound model. PLoS One. 2018; 13(9): e0203371.
  24.  Wu L, Xiong X, Wu X, Ye Y, Jian Z, Zhi Z, Gu L.. Targeting Oxidative stress and inflammation to prevent ischemia-reperfusion injury. Frontiers in Molecular Neuroscience, 2020; 13:28.
  25.  Mehrtash M, Mohammadi R, Hobbenaghi R. Effect of adipose derived nucleated cell fractions with chitosan biodegradable film on wound healing in rats. Wound Medicine, 2015; 10–11: 1–8.
  26.  Bodnar RJ. Epidermal Growth factor and epidermal growth factor receptor: The Yin and Yang in the treatment of cutaneous wounds and cancer. Advances in Wound Care, 2013; 2(1): 24-29.
  27.  Martin J, Zenilman M, Lazarus GS. Molecular microbiology: new dimensions for cutaneous biology and wound healing. Journal of Investigative Dermatology, 2010; 130: 38–48.