The aim of the present study was to assess the wound-healing activity of extract of Allium stipitatum. Thirty-six male Wistar rats were used in this study. The rats weighing approximately 160-180 g and seven weeks of age were randomized into three groups of 12 rats each: Control surgery group (Control) including the creation of wounds and no treatment, base formulation groups positive (POS) with the creation of wounds and application of base formulation ointment, treatment group 1 (T1) with 2 g of powder extract of the plant material in the ointment. A wound was induced by an excisional based wound model in male rats. The mature green leaves of Allium stipitatum were collected and authenticated. Extractions of dried leaves were carried out. For wound-healing activity, the extracts were applied topically in the form of ointment and compared to control groups. The healing of the wound was assessed based on the wound area, histomorphometry, and hydroxyproline estimation studies. Reduction in the wound area and hydroxyproline contents indicated that there was a significant difference (p = 0.001) between group T1 and other groups. Quantitative histological studies and mean rank of the qualitative studies demonstrated that there was a significant difference between group T1 and other groups (p = 0.001). The extract of Allium stipitatum leaves enhanced wound-healing activity significantly in both the wound models studied. Enhanced wound contraction, decreased epithelialization time, increased hydroxyproline content, improved histological characteristics studies suggested Allium stipitatum leaves extract might have therapeutic benefits in wound healing.