TY - JOUR ID - 92067 TI - Effects of Curcumin Nanoparticles on the Tissue Oxidative Stress Following Testicular Torsion and Detorsion in Rat Model JO - Iranian Journal of Veterinary Surgery JA - IVSA LA - en SN - 2008-3033 AU - Esmaeilsani, Zaman AU - Barati, Farid AU - Mohammadi, Rahim AU - Shams-Esfandabadai, Naser AU - Karimi, Iraj AD - Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran. AD - Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran. AD - Department of Pathobiology, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran. Y1 - 2019 PY - 2019 VL - 14 IS - 2 SP - 97 EP - 104 KW - ischemia-reperfusion KW - curcumin nanoparticle KW - testicular torsion DO - 10.22034/ivsa.2019.176105.1180 N2 - Objective-The aim of the present study was to investigate the effects of curcumin nanoparticles (CNP) on stress oxidative following experimental ischemia-reperfusion injury in the rat testes.Design-Experimental StudyAnimals- Seventy-seven healthy male Wistar ratsProcedures-The animals weighing approximately 250 g were randomly assigned to four experimental groups (n = 18): Ischemia: torsion group (created by 720° rotation of testis on both sides for 2 h). Ischemia/Reperfusion (I/R): torsion for 2 h followed distortion, CNP 10: received 10 mg/kg IP administration of CNP 30 min before surgery then remaining testes were twisted and untwisted, CNP 20: received 20 mg/kg IP administration of CNP 30 min before surgery then remaining testicles were twisted and untwisted. Unilateral orchiectomy of left or right testicles was performed on days 0 and 12 with immediately sampling. Some twisted then untwisted testicles were remained and sampled 60 days after surgery. An additional group was considered (n=5) to be sampled without any operation as control group. The samples of testicular tissue homogenates were taken on Days 0, 12 and 60, and their liquid extracts were collected and assayed for Superoxide dismutase (SOD), Malondialdehyde (MDA) and Glutathione peroxidase (GPX). The effect of treatment and day of sampling on the variables was analyzed using Two-Way ANOVA.Results- The results of the study showed an ameliorated balance of GPX, SOD and MDA following testicular torsion in this study. The CNP treated animals (with both administered doses) showed significantly improved the balance of the enzymes compared to untreated animals (p<0.0001).Conclusion and clinical relevance- In conclusion, IP administration of CNP may be helpful in minimizing oxidative stress related enzymes following testicular ischemia-reperfusion in rat. UR - https://www.ivsajournals.com/article_92067.html L1 - https://www.ivsajournals.com/article_92067_79843807937e5a1e8056aca1e17ddb12.pdf ER -