TY - JOUR ID - 50823 TI - In Vitro Evaluation of Equine Fibroblast-Like Synoviocytes Viability Treated with Doxycycline JO - Iranian Journal of Veterinary Surgery JA - IVSA LA - en SN - 2008-3033 AU - Ghasemi, Samaneh AU - Sardari, Kamran AU - Mirshokraei, Pezhman AU - Hassanpour, Hossein AD - Department of Clinical Sciences, School Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran. AD - Center of Excellence in Ruminant Abortion and Neonatal Mortality, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Razavi Khorasan, Iran AD - Department of Basic Sciences (Physiology Division), Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Chaharmahal and Bakhtiari, Iran Y1 - 2017 PY - 2017 VL - 12 IS - 1 SP - 11 EP - 17 KW - Doxycycline KW - Equine fibroblast-like synoviocytes KW - MTT assay KW - Trypn blue KW - viability DO - 10.22034/ivsa.2017.50823 N2 - Objective- The purpose of present study was to investigate the viability of equine fibroblast-like synoviocytes (FLSs) treated with doxycycline.Design- Experimental study.Sample population- FLSs from metacarpophalangeal joints of six skeletally mature horses.Methods- FLSs were established from synovial fluids of healthy joints. The cells were treated with different concentrations (1, 5, 10, 50, 100, 150, 300, 400 µg/ml) or without doxycycline for 48-hour. Viability of FLSs was determined by MTT assay and the Trypan blue dye exclusion method.Results- No significant differences were observed between viability of FLSs cultures treated with doxycycline until 150 µg/ml and control group (P>0.05). Doxycycline at 300 and 400 µg/ml significantly decreased FLSs viability (P<0.05). FLSs viability were 74.28% and 59.07% in 300 and 400 µg/ml, respectively, when measured by the MTT assay. Also FLSs viability at 300 and 400 µg/ml of doxycycline were 68.10% and 43.26%, respectively with Trypan blue exclusion method.Conclusion and Clinical Relevance- These findings demonstrate that doxycycline was not toxic for equine FLSs at concentration equal or less than 150 µg/ml in vitro. Further studies are needed to investigate the safety, efficacy and detrimental effects of doxycycline in equine joints. UR - https://www.ivsajournals.com/article_50823.html L1 - https://www.ivsajournals.com/article_50823_2dead6e226d813e8adf97f64c3e73a49.pdf ER -