TY - JOUR ID - 3181 TI - Growth Kinetics and in Vitro Aging of Mesenchymal Stem Cells Isolated From Rat Adipose Versus Bone Marrow Tissues JO - Iranian Journal of Veterinary Surgery JA - IVSA LA - en SN - 2008-3033 AU - Baghaban Eslaminejad, Mohamadreza AU - Mardpour, Soura AU - Ebrahimi, Marzieh AD - Department of Stem Cell and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran. AD - Department of Developmental Biology, University of Science and Culture, Tehran, Iran. Y1 - 2008 PY - 2008 VL - 03 IS - 2 SP - 9 EP - 20 KW - Mesenchymal Stem Cells KW - Adipose tissue KW - Bone Marrow KW - Growth Kinetics KW - senescence DO - N2 - Objective- To investigate and compare growth potential as well as aging of mesenchymal stem cells (MSCs) derived from rat bone marrow tissue and adipose tissue (AT) occurred at epicardial and epididymal regions. Design- Experimental study.   Animals- 10 Wistar Rats.   Procedures- Rat MSCs occurred at bone marrow and epicardial and epididymal AT were isolated and culture expanded through several successive passages. Differentiation potential along bone, cartilage and adipose cell lineages was used to verify MSC identity of the isolated cells, and then the cells were comparatively investigated in terms of their colonogenic ability, population doubling time and growth curve characteristics. Furthermore, the number of senescent cells at different passages was quantified using senescent- associated (SA) ß galactosidase staining method. Results- MSCs from both AT appeared to have more proliferation capacity in culture than those from bone marrow since they exhibited significantly more colony number and shorter PDT value (P<0.05). Epicardial AT-MSCs indicated even more significant proliferation capacity than their epididymal counterparts. With respect to cell aging, marrow-MSCs cultures indicated higher percentages of senescent cells than AT-MSCs (P<0.05). Although the percentages of senescent cells in epididmal AT-MSCs were higher than epicardial AT- MSCs but the difference was not statistically significant. Conclusion and Clinical Relevance- Taken together we concluded that rat epicardial AT- MSCs could be appropriate cells for experimental and preclinical settings since they possess more expansion rate and less percentages of senescent cells in culture. UR - https://www.ivsajournals.com/article_3181.html L1 - https://www.ivsajournals.com/article_3181_27ee2c078fcc7d80cfa35cefa9ff8046.pdf ER -