Iranian Veterinary Surgery Association (IVSA)
Iranian Journal of Veterinary Surgery
2008-3033
2676-6299
01
1
2006
04
01
Effect of Hydrocortisone on the Growth of cultured Equine Fibroblasts Isolated from Distal Aspects of the Limb.
7
14
EN
Omid
Azari
Department of Clinical Scinces, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran.
omid.azari@ut.ac.ir
Mehdi
Ghamsari
Department of Clinical Scinces, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran.
ghamsari@ut.ac.ir
Mohammad Mehdi
Dehghan
Department of Clinical Scinces, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran.
mdehghan@ut.ac.ir
Mohammad Reza
Aghcheloo
Department of Clinical Scinces, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran.
aghchelou@uoz.ac.ir
Objective- To determine the effects of the Hydrocortisone on in vitro growth of fibrobla sts isolatedfrom the distal aspects of the horses limbs.
Design- Experimental in vitro study.
Animals- A total of 4 Caspian miniature and 4 mixed thoroughbred horses.
Procedures- Under generalanesthesia and aseptic condition, a full thickness of skin incision was created on the lateral aspects of themetacarpal mid-third and 3 grams of subcutaneous tissue was harvested and placed on culture medium(RPMI-1640) in an incubator at 37°C in 5%C02. The Hydrocortisone was added in 3 different concentrations [treatm ent groups A (l014 g/ml) , B (100 14g/ml), and C (300 14g/ml)] into the culture medium and cellcounting was carried out after 4 days. The effects of Hydrocortisone added to control media were comparedwith a control media on fibroblast growth and viability. The data were analyzed by one way ANOVA andTukey test.
Results- There were no significant differences in fibroblast growth rate and viability percentage (p>O.05)between the groups control and A, but the groups Band C suppressed significantly the fibroblast growthand decreased viability percentage in compared with groups control and A (p<0.05), and also there were nosignificant differences between groups Band C in fibroblast growth rate and viability percentage (p>0.05).
Conclusion and Clinical Relevance- The effect of Hydrocortisone on cultured fibroblast growth isdose-dependant. Application ofthis agent can inhibit fibroblast growth; therefore it can be used for treatmentofexuberant granulation tissue and improving wound healing processes, especially in the distal limb woundsof the horses
https://www.ivsajournals.com/article_5097.html
https://www.ivsajournals.com/article_5097_7d1ee118ba3c65d9dfcb8ec6f01630cc.pdf
Iranian Veterinary Surgery Association (IVSA)
Iranian Journal of Veterinary Surgery
2008-3033
2676-6299
01
1
2006
04
01
Clinical Evaluation of Elective Laparoscopic Ovariohysterectomy in Dog
15
21
EN
Jalal
Bakhtiari
Department of Clinical Studies, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran.
bakhtiar@ut.ac.ir
Sarah
Mokaram
Department of Clinical Studies, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran.
sarah.mokaram@gmail.com
Alireza
Khalaj
Department of surgery, Faculty of medicine, University of Shahed, Tehran,Iran.
akhalaj@yahoo.com
Davood
Sharifi
Department of Clinical Studies, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran.
dsharifi@ut.ac.ir
Azin
Tavakoli
Department of Clinical Studies, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran.
azin.tavakoli@gmail.com
Objectives- To introduce laparoscopic ovariohysterectomy in dog in Iran,
Study Design- Elective experimental study design.
Animals- Four mix-breed healthy female dogs.
Methods- Four healthy female dogs were undergone elective laparoscopic ovaryohysterectomy. Clinical parameters including: body temperature, heart rate, respiratory rate, appetite, surgical time, blood loss, surgical complications, CBC and pain scores were evaluated.
Results- CBC of the dogs were not indicator of infection but the stress leuckogram was evident till 48 hours after the surgery. Median pain scores 2 and 4 hours after the surgery were low (pain scores less than 6) and 24 hours post operatively were very low (pain scores less than 2). The blood loss was minimumand the operations had no complications. Appetite, body temperature, respiratory and heart rate returned tonormal values in a short period (48 hours).
Conclusions and Clinical Relevance- Laparoscopic ovariohysterectomy is safe and could be performedin a reasonable time with minimal pain in dogs.
https://www.ivsajournals.com/article_5099.html
https://www.ivsajournals.com/article_5099_a0a31a5badaba5f56753213bcd601f42.pdf
Iranian Veterinary Surgery Association (IVSA)
Iranian Journal of Veterinary Surgery
2008-3033
2676-6299
01
1
2006
04
01
The Use of Tail Vertebra as Cortical Bone Graft in Experimentally Induced Ulnar Defects in Dogs
22
30
EN
Ali
Baniadam
Department of Surgery,Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran.
abaniadam@gmail.com
Fereidoon
Saberi Afshar
Department of Surgery,Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran.
saberiafshar@ut.ac.ir
Ali Reza
Ghadiri
Department of Surgery,Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran.
alighadiri@scu.ac.ir
Zahra
Karimi Jalalabadi
Graduated from the Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran.
Objective- To evaluate tail vertebra as an autogenous cortical bone and cancellous bone for filling ulnarsegmental bone defect in dogs.
Design- Experimental study.
Animals- Eight adult mongrel dogs with an average age of 37.S months and average body weight of17.62 kg.
Procedure- The animals were randomly divided into two groups. A bone defect (2.S-cm) was created on the middle portion of the right ulna in all dogs. Ulna was fixed by intramedullary pinning. In 5 dogs (test group) ulnar bone defects were filled with tail vertebra, after tail docking. Cancellous bone harvested fromthe il iac cres t was also used to promote bone healing in the ho st-graft inter face s. In the other 3 dogs(control group) ulnar bone defect s were not filled. Po stoperative clinical assessment was includ edmeasurement of body temperature, heart rate, respiratory rate, and evaluation of the degree of lameness. Radiographic assessment of bone based on the process of bone healing and consolidation of the host-graft interface, periosteal reaction, and soft tissue swelling were performed immediately and at the weeks 1, 3, S,7, 10, 13 and 17 after surgery.
https://www.ivsajournals.com/article_5107.html
https://www.ivsajournals.com/article_5107_d5d1b059e9d6a833f55a34da33f0f9e4.pdf
Iranian Veterinary Surgery Association (IVSA)
Iranian Journal of Veterinary Surgery
2008-3033
2676-6299
01
1
2006
04
01
Intrasplenic Autotransplantation of Hepatocytes in Dogs
31
35
EN
Amin
Bigham Sadegh
0000-0003-4981-7495
Department of Veterinary Surgery, Shiraz University, Shiraz, lran.
dr.bigham@gmail.com
Seifollah
Dehghani
Department of Veterinary Surgery, Shiraz University, Shiraz, lran.
sdehghan04@yahoo.com
Zahra
Shafiei Sarvestani
Student of Veterinary Surgery, Shiraz University, Shiraz, lran.
dr.shafiei.m@gmail.com
Objective- The liver has a unique location in the body for performing multiple functions such as, metabolism, detoxification and synthesis. There are limitation for liver transplantation due to shortage of available donors and requires for sophisticated technology and support surgical teams. The purpose of this study was to investigate the hepatocyte transplantation into the spleen of animal model such as dog to establish basic condition for further studies.
Design- In vivo experimental Study.
Animals- Five Iranian indigenous mixed breed dogs of both sexes, 15/8±3 months old and 19.6±8.2weighting were used in this study.
Procedures- Ventral midline celiotomy was performed under general aneasthesia and liver sampls were obtained using suction technique by a 50cc syringe and 14 gauge needle. Then obtained samples were injected into the same dog 's spleen directly and also via splenic artery, the celiotomy incision sutured and the animal left to recover. On the 16th postoperative day, under general anaesthesia, the gross appearance of spleen were recorded and photographed and for histopathological evaluation, partial splenectomy was performed. The prepared sections were stained with Hematoxylin Eosin [H& E] and Periodic Acid Schiff[PAS] and studied under light microscope.
Results- The gross evaluations of the removed spleen showed a whitish area at the injection site in all of the 5 transplanted dogs, spleen vessels had been dilated and enlarged in diameter. Microscopically the groups of hepatocytes in the center of lymphoid follicles in the white pulp were noted.
Conclusion and Clinical Relevance- Hepatocyte transplantation could be useful for both supporting an acute liver failure and for surviving as a bridge to liver transplantation in terminal liver failure.
https://www.ivsajournals.com/article_5110.html
https://www.ivsajournals.com/article_5110_8adeb534df6a966b9a21ab00fc79fab3.pdf
Iranian Veterinary Surgery Association (IVSA)
Iranian Journal of Veterinary Surgery
2008-3033
2676-6299
01
1
2006
04
01
Histopathological and Biomechanical Study on the Effect of Aftemisia sieberi Extract on Experimental Skin Wound Healing in Rat
36
42
EN
Amin
Derakhshanfar
Departmentof Pathobiology,Faculty of Veterinary Medicine, Shahid Bahonar university of Kerman, Kerman, Iran.
derakhshanfar@sums.ac.ir
Mohammad Mehdi
Oloumi
Departmentof Clinical Studies,Faculty of Veterinary Medicine, Shahid Bahonar university of Kerman, Kerman, Iran.
oloumi.m@gmail.com
Jahangir
Kabootari
Department of Physiology and Pharmacology, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran.
Hossein
Ali Arab
Department of Physiology and Pharmacology, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran.
Objective- To evaluate the effects of Artemisia sieberi extract on experimental dermal wounds healing.
Design- Experimental study.Animals- 30 male Spragne-Dawly rats.
Procedures- Two uniform 7 mm diameter skin defect were created on the back of each animal by skinpunch (total of 60 wounds). Then, the extract of the plant was applied once daily on half of the wounds forhistopathological and biomechanical examinations.
Results- Artemisia sieberi extract caused a significant increase in the number of fibroblasts and capillarybuds and also significant decrease in the epithelial gap which showed the better formation of healing tissuein treatment group. On the other hand, the improvement of biomechanical indices in treatment group revealed a significant increase in tensile strength of the wounds.
Conclusion and Clinical Relevance- It can be concluded that Artemisia sieberi is an effective herbalremedy in wound healing.
https://www.ivsajournals.com/article_5111.html
https://www.ivsajournals.com/article_5111_e0037c4033aea75814c838e2a6686e6d.pdf
Iranian Veterinary Surgery Association (IVSA)
Iranian Journal of Veterinary Surgery
2008-3033
2676-6299
01
1
2006
04
01
Normal Color and Pulsed-Waved Doppler Ultrasonography of Femoral and Axillary Arteries in Dogs
43
49
EN
Majid
Masoudifard
Department of Clinical Scinces, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran.
mmfard@ut.ac.ir
Alireza
Vajhi
Department of Clinical Scinces, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran.
avajhi@ut.ac.ir
Ali
Kavari
Private Veterinary Practitioner
Mohammad Hadi
Borhani Haghighi
Private Veterinary Practitioner
Objective- Obtaining the normal images of color doppler and estimation of blood flow velocity parameters of femoral and axillary arteries in dog.
Design- Descriptive studyAnimals- 7 healthy adult mixed-breed dogs, weighing 14.3 ± 2.8 kg (mean ± SD).
Proceudure- Left and right femoral and axillary arteries of hind and forelimbs were evaluated. Color and spectral doppler images of the artery were obtained. Values of peak systolic velocity, end-diastolic velocity, mean velocity, resistive index and pulsatility index were measured. Statistical analysis- Calculation of mean and standard deviation of measured parameters using Microsoft excel 2003.
Results- Color Doppler of both arteries showed a laminar flow with higher velocity in the center of flow. High-resistance flow pattern with triphasic flow velocity and plug velocity profile were observed in spectral waveform analysis of pulsed Doppler.
Conclusion and Clinical Relevance- Results of this study can be useful for hemodynamic evaluation of femoral and axillary arteries diseases such as thromboembolism and arterial stenosis in dogs.
https://www.ivsajournals.com/article_5222.html
https://www.ivsajournals.com/article_5222_748b350651d02de1ac07f2b283814965.pdf
Iranian Veterinary Surgery Association (IVSA)
Iranian Journal of Veterinary Surgery
2008-3033
2676-6299
01
1
2006
04
01
Use of Conjunctival Grafts and Temporary Tube Implants for Treatment of Teat Sinus Mucosal Injuries
50
57
EN
Mohammad Mehdi
Molaei
Department of Clinical Science, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
molaei_mm@mail.uk.ac.ir
Jalil
Abshenas
Department of Clinical Science, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
jabshenasm@yahoo.com
Amin
Derakhshanfar
Department of Pathobiology,Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
derakhshanfar@sums.ac.ir
Homayoon
Babaei
Department of Clinical Science, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
babaei_h@uk.ac.ir
Objectives- Using conjunctival graft to replace experimental mucosal defects of teat sinus.
Study- Experimental study.
Animals- Five healthy, mature, lactating, Jersey cows.
Methods- Conjunctival mucosal grafts with teat cannula were evaluated in the repair of experimental teat sinus mucosal lesions. A 1.5xl em wide circumferential strip of mucosa was resected from each teat in five Jersey cows. Each lesion was then treated by one of the three methods: 1. teat cannula (C ); 2. Conjunctivalmucosal graft and teat cannula (C+G); 3. Only mucosal graft (G). All teats were evaluated 4 weeks after treatment, for patency and luminal diameter by radiography and ultrasonic examination. The cow s were euthanized, and teats were collected for histopathologic study.
Results- The histopathologic and radiographic results showed that the conjunctival tissue of the teat was incorporated by the teat mucosal tissue. Some acanthosis in conjunctiva was observed, due to the stimulation of the teat cannula.
Conclusion and Clinical Relevance- Conjunctival grafts can be used in teat mucosal defect s when other therapeutic methods fail to keep the integrity of the teat sinus mucosa.
https://www.ivsajournals.com/article_5223.html
https://www.ivsajournals.com/article_5223_c691cbda8d369982bc2b9b1c4919aea7.pdf
Iranian Veterinary Surgery Association (IVSA)
Iranian Journal of Veterinary Surgery
2008-3033
2676-6299
01
1
2006
04
01
Enhancement of Bone Healing by Static Magnetic Field in the Dog: Biomechanical Study
58
64
EN
Siamak
Saifzadeh
Department of Clinical Scinces, College of Veterinary Medicine, Urmia University, Urmia, Iran.
s.saifzadeh@mail.urmia.ac.ir
Ghader
Rezazadeh
Department of Mechanics, College of Engineering, Urmia University,Urmia . Iran
Bahram
Dalir Naghadeh
Department of Clinical Scinces, College of Veterinary Medicine, Urmia University, Urmia, Iran.
b.dalir@urmia.ac.ir
Mahsa
Ajodani
Graduated from the college of Veterinary Medicine, Urmia University, Urmia, Iran.
Objective- Although the promotional effects on bone healing of pulsed electromagnetic fields (PEMF)have been well demonstrated, the effects of static magnetic fields (SMF) remained unclear. In this study, effects of SMFs on clinical and biomechanical aspects of bone healing using a canine unstable osteotomy gap model were investigated.
Design- Prospective descriptive trial. Animals- Fifteen mongrel dogs, 4 to 5 years old and weighing 15.5 to 21.3 kg.
Procedures- After an osteotomy of the midshaft radius, bone healing was evaluated over an 8-week period in control dogs (n = 5) and experimental dogs exposed to medium (700 gauss) (MSMF) and high (1500 gauss) (HSMF) magnetic fields (n = five/each group). Bone healing was assessed clinically and biomechanically.
Results- Dogs exposed to HSMF had more improved lameness scores in contrast to the MSMF and control dogs. Significantly, greater forces
https://www.ivsajournals.com/article_5225.html
https://www.ivsajournals.com/article_5225_56f08e4cde6c159373488706e2fe7318.pdf
Iranian Veterinary Surgery Association (IVSA)
Iranian Journal of Veterinary Surgery
2008-3033
2676-6299
01
1
2006
04
01
Arthroscopic Findings Following Experimental Cranial Cruciate Ligament Desmotomy in Dog
65
72
EN
Kamran
Sardari
Department ofClinical Sciences. Faculty of Veterinary Medicine. Ferdowsi University of Mashad. Mashhad. lran
sardari@um.ac.ir
Ahmadreza
Raji
Department of Basic Sciences. Faculty of Veterinary Medicine. Ferdowsi University of Mashad. Mashhad. lran
rajireza@ferdowsi.um.ac.ir
Nazanin
Farazan
Veterinary Student. Faculty of Veterinary Medicine. Ferdowsi University of Mashad. Mashhad. lran
Objective- Using the arthroscopic surgery technique for experimental transection of cranial cruciate ligament and arthroscopic findings of stifle joint following ligament transection.
Design- Experimental study.
Animals- Five healthy mixed breed dogs.
Procedures- Five dogs (mean weight 27±3 kg, mean age 3 years) selected to study the stifle joint changes after experimental cranial cruciate ligament desmotomy. The cranial cruciate ligament of the one stifle joint was transected via arthroscopy in all dogs under general anesthesia. One month after cranial cruciate ligament desmotomy the arthroscopic examination of the joints was performed again. Lateral and antrioposterior radiography were also carried out before transected of the ligament and one month after ligament surgery just before second arthroscopy.
Results- All dogs had various degree of lameness during the study and showed inflammatory changes in the joint. In all animals arthroscopic observation showed various degrees of the articular cartilage damage and meniscal injuries.
Conclusion and Clinical Relevance- According to the results of present study, the cranial cruciate ligament is vital to normal function and rupture of this ligament result s in progressive and permanent degenerative changes within the joint.
https://www.ivsajournals.com/article_5226.html
https://www.ivsajournals.com/article_5226_d7c22618d30b159d0e3d1e09814b9dc2.pdf
Iranian Veterinary Surgery Association (IVSA)
Iranian Journal of Veterinary Surgery
2008-3033
2676-6299
01
1
2006
04
01
A Comparison Between the Effects of Xylazine-Ketamine and Xylazine-Thiopental Combinations on Cardiac Rhythm in Dogs
73
81
EN
Bahram
Dalir-Naghadeh
Department of Clinical Sciences, College of Veterinary Medicine, Urmia University, Urmia, Iran.
b.dalir@urmia.ac.ir
Farshid
Sarrafzadeh-Rezaei
Department of Clinical Sciences, College of Veterinary Medicine, Urmia University, Urmia, Iran.
f.sarrafzadeh@mail.urmia.ac.ir
Loghman
Mobaraki
Graduated in College of Veterinary Medicine, Urmia University, Urmia, Iran.
Goudarz
Sadeghi-Hashjin
Department of Basic Sciences, College of Veterinary Medicine, Urmia University, Urmia, Iran.
Objective- To compare the effects of xylazine-ketamine and xylazine-thiopental on cardiac rhythm in dogs.
Design- Experimental study.
Animals- 15 mixed-bred, adult dogs of either sex.
Procedures- Following premedication with xylazine HCI, either ketamine HCI or sodium thiopental were administered to dogs. Cardiac rhythm was evaluated before as well as 15,30,45, and 60 minutes and3 days after induction of anesthesia.
Results- Sinus arrest block and first degree atrioventricular (A-V) block were the most common arrhythmias observed after ketamine administration. During thiopental-induced anesthesia, ventricular premature beats with bigeminy pattern and sinus bradycardia were also recorded.
Conclusion and Clinical Relevance-Concerning the documented effects of the aforementioned drugs on cardiac rhythm, arrhythmias might be induced by the combination of xylazine with both anesthetic drugs.
https://www.ivsajournals.com/article_5228.html
https://www.ivsajournals.com/article_5228_4970805bdf818c9f6c00da1b969868d2.pdf
Iranian Veterinary Surgery Association (IVSA)
Iranian Journal of Veterinary Surgery
2008-3033
2676-6299
01
1
2006
04
01
Possibility and Reproducibility of Renal Assessing and Size Measurement by Three-Dimensional vs Two-Dimensional Ultrasonography in Dogs
82
87
EN
Mohammad
Molazem
Department of Clinical Scinces, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran.
mmolazem@ut.ac.ir
Alireza
Vajhi
Department of Clinical Scinces, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran.
avajhi@ut.ac.ir
Majid
Masoudifard
Department of Clinical Scinces, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran.
mmfard@ut.ac.ir
Daroush
Vosough
Department of Clinical Scinces, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran.
dvosugh@yahoo.com
Objective- To determine the possibility and reproducibility of three-dimensional ultrasonography (3DUS) and comparison of the achieved measurements to normal two-dimensional ultrasonography (2DCS).
Design- Descriptive study.
Animals- 10 young mixed normal dogs, age 1.5-2.5 year, weighing 9.7-12 kg.
Procedure- Renal width, length, and depth measured in coronal and transverse sections. The measurements were taken in both 2DUS and 3DUS scans. Each kidney was imaged five time s in coronal and transverse section and the measurements were recorded for each image. Statistical analysis-Descriptive analysis, paired t-test.
Results- The mean difference of the measurements obtained by 3DUS in compare with 2DUS was: 0.17em for renal length, 0.08 ernforrenal width, and 0.13 em for renal depth. There was no significant difference between 3DUS and 2DUS.
Conclusion and Clinical Relevance- In this study the correlation was seen between 3DUS and 2DUS.The3DUS method gives us extremely useful images for teaching and diagnosing purposes. The 3DUS image presentation can also be easily demonstrated in rotating 3Danimation.
https://www.ivsajournals.com/article_5231.html
https://www.ivsajournals.com/article_5231_c60cdaef0cb084117dae49ec0d40c355.pdf