%0 Journal Article %T Equine Bone Marrow Derived Mesenchymal Stem Cells: Isolation and Multilineage Differentiation %J Iranian Journal of Veterinary Surgery %I Iranian Veterinary Surgery Association (IVSA) %Z 2008-3033 %A Azad, Elnaz %A Adibhashemi, Farajollah %A Dehghan, Mohammad Mehdi %A Ghamsari, Seyed Mehdi %A Sedaghat, Reza %A Nasiri, Seyed Mehdi %A Abbasnia, Pegah %D 2014 %\ 06/01/2014 %V 09 %N 1 %P 45-52 %! Equine Bone Marrow Derived Mesenchymal Stem Cells: Isolation and Multilineage Differentiation %K Equine %K Bone Marrow %K Mesenchymal stem cell %R %X Objective- To evaluate growth characteristics and differentiation capacity of equine mesenchymal stem cell (eMSCs) derived from bone marrow (BM). Study design- In vitro experimental study. Animals- Four young adult horses (2-5 years old) Procedure- Cell morphology and growth characteristics of eMSCs harvested from BM were evaluated in standard culture conditions. eMSCs in passage 3 were subjected to osteogenic and adipogenic differentiation induction to investigate their differentiation potential.PCR analysis of differentiated cells was done to determine differentiation. Results-The cells expanded to sufficient quantity for therapeutic purposes within days and they survived to later passages while sustained their fibroblast-like morphology. Positive osteogenesis was detected via Alizarin Red staining and adipogenesis was confirmed by Oil Red O staining. The cells were more potential to differentiate into osteoblasts rather than adipocytes. PCR analysis approve relative expression of osteogenic and adipogenic genes. Conclusions and Clinical relevance - The results of this study further support eBM-MSCs as a cell population with the capacity to proliferate and differentiate down the osteogenic and adipogenic lineages. Equine BM is a potentials source of MSCs for cell-based regenerative therapies in horse.     %U https://www.ivsajournals.com/article_5906_de17157fe76e618d5dd5d2d99ed1bd37.pdf